关于pMP7017大质粒的转录景观及其对Bifidobacterium breve UCC2003转录组的影响
Rebecca L Dineen1,2, Francesca Bottacini2,3, Mary O'Connell-Motherway1
1APC Microbiome Ireland, University College Cork, Cork, Ireland.
Microbial biotechnology
|January 11, 2024
概括
已知最大的Bifidobacterium等离子体,pMP7017,具有很高的转录率,并影响宿主基因表达,特别是在遗传信息处理和生物膜生产中. 这种巨质粒在婴儿和母亲的微生物组中广泛存在.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 双杆菌物种是关键的肠道食用动物.
- 巨质粒是大型DNA分子,可以为它们的宿主细菌带来显著的优势.
- 在Bifidobacterium breve中的pMP7017等离子体是这个属中最大的和第一个特征的结合性等离子体.
研究的目的:
- 为了研究pMP7017大质的生物学.mid.
- 了解等离子体载体对宿主Bifidobacterium菌株的影响.
- 评估pMP7017和相关等离子体的流行和进化起源.
主要方法:
- 综合方法结合了转录学,全基因组比较分析和元基因组数据挖掘.
- 促进子元素和基因转录的分析.
- 对具有或没有等离子体的宿主菌株进行了codon使用概况分析和比较转录组分析.
主要成果:
- pMP7017表现出高的转录活性,编码适应系统和tmRNA.
- 对比分析揭示了相关等离子体中基因组织和辅助基因的显著多样性.
- 大基因组数据表明,pMP7017同类物体存在于~10%的人类微生物组样本中.
- 等离子体载体显著改变了 ~8% 的宿主 B. breve UCC2003 基因的表达,包括那些参与遗传信息处理,生物膜生产和多糖生物合成的基因.
结论:
- pMP7017是一种转录活性的大质粒,对宿主基因表达有显著影响.
- 这种等离子体很可能起源于Bifidobacterium longum,并且在婴儿和母亲的微生物组中普遍存在.
- 等离子体载体影响宿主的遗传信息处理和代谢能力,可能影响宿主-微生物相互作用.
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