在含有维生素C的盐水储存溶液中保存的的表面特性和体外生物相容性
Wen-Si Zhang1,2, Yao Liu1,2, Shui-Yi Shao1,2
1Department of Oral Implantology, Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, PR China.
Journal of materials science. Materials in medicine
|January 11, 2024
概括
在100mM维生素C盐溶液中储存植入物可以减少碳污染,提高水友性. 这增强了骨质细胞的粘附,增殖和分化,以更好地整合骨.
科学领域:
- 生物材料科学 生物材料科学
- 表面化学 表面化学
- 整形外科植入物 整形外科植入物
背景情况:
- 植入物在骨科中至关重要.
- 在存储期间保持植入物表面完整性对于骨质整合至关重要.
- 碳污染和表面疏水性可能会阻碍植入物性能.
研究的目的:
- 评估维生素C (VitC) 盐溶液作为植入物的储存介质.
- 调查VitC储存对表面性能和骨质生成潜力的影响.
主要方法:
- 商业纯 (cp-Ti) 和SLA-Ti表面被存储在空气,盐水,10mM VitC或100mM VitC盐水中2周.
- 表面分析包括SEM,拉曼,XPS,水接触角度和粗度测量.
- 评估了蛋白质吸附和MC3T3-E1骨质细胞反应 (粘附,增殖,骨质分化).
主要成果:
- 与空气或盐水储存相比,100mM VitC储存减少了碳污染,增加了表面的水友性.
- 储存VitC增强了对表面的蛋白质吸附.
- 在100mM VitC盐溶液中储存后,骨质细胞粘附,增殖和骨质原生标志物的表达显著上调.
结论:
- 在100mM VitC盐水中保存cp-Ti和SLA-Ti有效地减少了碳污染,并增强了表面的水友性.
- 这种储存方法促进了骨质细胞的反应,表明植入物具有改善的骨质生成潜力.
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