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替代拼接揭示了Litopenaeus vannamei在高性的急性压力反应
Xiang Shi1, Ruiqi Zhang2, Zhe Liu1
1College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Gansu Province, Lanzhou, 730070, China.
Marine biotechnology (New York, N.Y.)
|January 11, 2024
概括
替代拼接 (AS) 是面临高性的的关键应激反应. 这项研究揭示了AS机制,并确定了在性压力下对免疫力至关重要的拼接因素.
科学领域:
- 水生物质毒理学研究
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 性对盐水环境中的水生生物造成重大压力.
- 替代拼接 (AS) 增强了转录多样性,对应激适应至关重要.
- 关于甲类动物对性压力的AS反应的研究有限.
研究的目的:
- 在急性性压力下调查太平洋白 (Litopenaeus vannamei) 的AS事件.
- 确定关键的基因,拼接因子和性应激反应中的途径.
- 在高性条件下阐明AS在免疫力中的作用.
主要方法:
- 用RNA测序 (RNA-seq) 来比较在控制 (50 mg/L) 和高性 (350 mg/L) 条件下的.
- 对替代拼接事件和差异替代拼接 (DAS) 事件的分析.
- 功能性注释和差异拼接基因和已识别的拼接因子的途径分析.
主要成果:
- 总共有10,556个AS事件和619个DAS事件被确定.
- 不同拼接的基因主要参与了拼接体.
- 五个拼接因子 (SFs) 被上调,促进AS,并激活了白细胞超内皮细胞迁移和mTOR信号传递等途径.
结论:
- 替代拼接是太平洋白响应急性性压力的关键调节机制.
- 拼接因素在AS中起着至关重要的作用,HSFP1,SRSF2和NHE-RF1等特定的SF正在经历AS.
- 免疫相关基因中的差异性AS是应对性压力的重要适应,为类应对压力提供了新的见解.
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