抗氧化系统的改变,氧化和基因毒性影响在丹尼奥雷里奥 (斑马鱼) 暴露在垃圾填埋场的液中
Sidney Fernandes Sales Junior1,2, Nicolle Martins da Costa1, Gabriel de Farias Araújo1,2
1Post-graduation Program in Public Health and Environment, Sergio Arouca National School of Public Health, Oswaldo Cruz Foundation, 1480 Leopoldo Bulhões Ave, Rio de Janeiro, RJ, 21041-210, Brazil.
Environmental science and pollution research international
|January 11, 2024
概括
从垃圾填埋场的液污染了水,伤害了斑马鱼. 暴露会改变它们的抗氧化系统,增加氧化应激,并导致DNA损伤,突出了城市固体废物管理中的风险.
科学领域:
- 环境毒理学环境毒理学
- 水生生态毒理学 水生生态毒理学
- 生物标志物研究 生物标志物研究
背景情况:
- 垃圾填埋场的液对水体和水生生物构成重大威胁.
- 城市固体废物管理需要了解水的生态毒理影响.
- 丹尼奥雷里奥 (斑马鱼) 是一个敏感的模型生物体,用于评估水中污染物的影响.
研究的目的:
- 为了评估垃圾填埋场液对斑马鱼 (Danio rerio) 的抗氧化系统的影响.
- 评估斑马鱼暴露于不同度的液中的氧化和基因毒性损伤.
- 为了确定抗氧化剂防御对液诱导的压力的有效性.
主要方法:
- 斑马鱼在96小时内暴露在5,15,30和50%的水度下.
- 评估肝脏和大脑酶活性 (超氧化物脱酶,触酶,谷氨-S转移酶) 和生物标志物度 (减少谷氨,金属氨).
- 测量了氧化应激标志物 (甲,蛋白质碳化) 并使用彗星测定评估了血液基因毒性.
主要成果:
- 浸出物暴露显著改变了斑马鱼肝脏和大脑中的抗氧化酶活动和生物标志物水平.
- 观察到氧化应激标记的增加 (蛋白质碳化) 和显著的基因毒性 (橄尾时刻的增加).
- 主要成分分析表明抗氧化防御不足以防止活性氧物种的积累和随后的损伤.
结论:
- 垃圾填埋场的液诱导斑马鱼的亚致命作用,破坏抗氧化剂系统并引起氧化应激和基因毒性.
- 目前的抗氧化剂反应不足以防止液诱导的损伤.
- 对暴露于多种液的斑马鱼进行进一步的研究对于在城市固体废物管理中进行可靠的风险评估至关重要.
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