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在 Vitro 中,使用分子程序进行酶自我选择

Adèle Dramé-Maigné1, Rocío Espada1, Giselle McCallum1

  • 1Gulliver UMR CNRS 7083, ESPCI Paris, Université PSL, 75005 Paris, France.

ACS synthetic biology
|January 11, 2024
PubMed
概括

我们开发了一种用于酶工程的新体外自我选择方法,将酶功能与基因复制联系起来. 这种方法可以对数百万个变异进行高通量选,以提高酶活性和稳定性.

关键词:
生物催化剂的生物催化剂化学反应网络的化学反应网络.指导进化是指导进化的.酶是一种酶.在体外 (in vitro) 的情况下.微流体学 在微流体学方面分子编程是分子编程.超高吞吐量选择的选择

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Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
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