哺乳动物的ortoreovirus可以在细胞外囊中退出细胞
Sydni Caet Smith1, Evan Krystofiak2, Kristen M Ogden1,3
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
PLoS pathogens
|January 11, 2024
概括
雷奥病毒通过细胞外囊泡 (EV) 和自由颗粒离开细胞. 中等EVs保护reovirus免受抗体中和,这取决于病毒菌株和细胞类型,使多颗粒感染.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 病毒利用各种退出途径,包括细胞外囊泡 (EVs) 进行非性释放.
- 通过EV介导的病毒输出可以促进免疫逃避和协调的病毒传播.
- 奥尔托雷病毒 (reovirus) 菌株表现出不同的细胞溶解效率,但退出机制仍被部分理解.
研究的目的:
- 调查reovirus出口机制和EVs在病毒传输和感染性中的作用.
- 为了确定reovirus菌株T1L和T3D是否利用EVs进行细胞退出.
- 评估EV协会对reovirus中和抗性和多颗粒感染的影响.
主要方法:
- 鼠类纤维细胞 (L细胞) 和人类结肠上皮细胞 (Caco-2) 感染了reovirus菌株T1L和T3D.
- 对不同大小的EVs (大,中,小) 和自由的reovirus进行细胞培养超级浸泡剂的丰富.
- 评估EV相关的reovirus感染性和对抗体介导中和的保护.
- 使用遗传编码的reovirus来追踪通过EVs的粒子运输.
主要成果:
- 两种reovirus菌株T1L和T3D都与大型和中型EV以及自由颗粒相关.
- 含有REOVIRUS的EV丰富分数具有传染性.
- 中等EVs提供了对抗体中和的保护,这种中和是特定于reovirus菌株和细胞类型的.
- 基因条形编码的reovirus表明,大型和中型EV可以将多个病毒颗粒传递给受体细胞.
- 雷奥病毒感染增加了所有EV大小从L细胞的释放.
结论:
- 雷奥病毒采用自由颗粒释放和与大型和中型EV结合的方法,以便从不同类型的细胞中退出.
- EV介导的退出可以导致多颗粒感染,并提供对抗体中和的部分保护.
- 电子病毒对中和的保护作用取决于上下文,因病毒菌株和宿主细胞而异.
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