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在Mycolicibacterium smegmatis的休眠细胞中甲基化氨酸的积累伴随着膜流动性的减少和呼吸链的功能受阻

Ivan A Gligonov¹, Daria I Bagaeva¹, Galina R Demina¹

¹A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Moscow 119071, Russia.

Biochimica et biophysica acta. Biomembranes

|January 11, 2024

概括

休眠的细菌会积累四甲基甲 (TMC),减少膜流动性并抑制呼吸活动. 这种机制有助于菌根细菌进入低代谢状态.

科学领域:

微生物学微生物学
生物化学生物化学
细胞生物学细胞生物学

背景情况:

结核菌和MSM过渡到休眠状态.
休眠期涉及细胞壁组成和代谢活动的变化.
氨酸与细菌生理学有关.

研究的目的:

为了研究四甲基甲 (TMC) 在菌根细菌休眠中的作用.
了解TMC积累对膜流动性和呼吸功能的影响.
阐明转化到低代谢状态的基础机制.

主要方法:

与焦点共聚显微镜测量BODIPY FL C16.的光异构性.
在Mycobacterium smegmatis (Msm) 细胞中量化TMC.
测量氧气消耗和DCPIP氧化还原受体活性.
使用5 - 氨基诺列维林酸诱导氨酸合成.

主要成果:

休眠的MSM细胞显示TMC积累增加和膜流动性改变 (光异性变异性从0.05增加到0.22).
植物细胞中TMC度的增加也导致了更高的异性质.
TMC积累与抑制呼吸链活动和减少DCPIP减少相关.
在重新激活后,TMC水平和膜流动性在8小时内恢复到植物细胞水平.

结论:

在休眠期间TMC在膜中的积累会降低膜流动性,抑制呼吸链活动.
这种膜变化可能有助于休眠菌根菌的低代谢状态.
TMC与膜结合酶的直接相互作用也是可能的贡献者.

关键词:

同焦点显微镜的共聚焦显微镜休眠的真菌菌菌是什么?光异质性是一种光异质性.膜流动性膜流动性甲基化甲基氨酸氨酸.

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