交配生活方式由Arabidopsis ROS和细菌T2SS之间的负反循环调节
Frederickson Entila1,2, Xiaowei Han1,3,4, Akira Mine5,6
1National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Nature communications
|January 11, 2024
概括
植物免疫利用活性氧物种 (ROS) 控制潜在的有害细菌. 这种机制服了叶子共生物,将其转化为阿拉比多普西斯植物的有益微生物.
科学领域:
- 植物与微生物的相互作用
- 植物免疫力 植物免疫力
- 微生物的病原发生.
背景情况:
- 植物微生物群可以包含有益和有害的微生物.
- 植物免疫力选择性控制其微生物群的机制在很大程度上是未知的.
研究的目的:
- 研究植物免疫力,特别是反应性氧物种 (ROS) 生产如何塑造植物微生物群.
- 了解NADPH氧化酶RBOHD在控制共生细菌中的作用.
主要方法:
- 从阿拉比多普西斯植物中分离和描述共生细菌.
- 对由共生细菌触发的ROS生产模式的分析.
- Xanthomonas L148的随机突变发生,以确定涉及致病性的基因.
- 在植物细菌转录组学研究基因表达.
主要成果:
- 结合性细菌以免疫受体和RBOHD依赖的方式触发ROS的产生.
- RBOHD可以选择性地抑制特定的共生物,包括Xanthomonas L148.
- 在L148的gspE基因突变,编码II型分泌系统 (T2SS) 组件,废除其对rbohD突变植物的有害影响.
- RBOHD抑制了细菌T2SS基因表达,包括gspE.
- 通过ROS或突变抑制gspE使L148对细菌病原体产生保护作用.
结论:
- 在Arabidopsis ROS和细菌T2SS之间存在负反循环.
- 这种循环服潜在有害的叶子共生菌,将它们转化为有益的微生物.
- 通过RBOHD介导的ROS生产对于维持有益的植物微生物群至关重要.
相关概念视频
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:26Defenses Against Pathogens and Herbivores
23.7K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
23.7K
01:10Transcription
147.0K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.0K
01:07Cell Signaling Feedback Loops
6.3K
Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
6.3K
01:30Bacterial Signaling
32.2K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.2K
01:31Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K