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调节肠道微生物群的益生菌联盟无菌地中海果的组成和功能

Hamden Haytham¹, Charaabi Kamel¹, Djobbi Wafa¹

¹Laboratory of Biotechnology and Nuclear Technologies, LR16CNSTN01, National Centre of Nuclear Sciences and Technologies, Sidi Thabet, Tunisia.

Scientific reports

|January 11, 2024

概括

益生菌联盟 (LEK-PC) 改善了无菌地中海果的健康状况,并恢复了肠道微生物群. 这提高了无菌昆虫技术 (SIT) 的害虫防治.

科学领域:

昆虫学昆虫学是一门学科.
微生物学微生物学
虫害管理虫害管理虫害管理

背景情况:

无菌昆虫技术 (SIT) 对于害虫控制是有效的,但由于大规模养殖和辐射,可以降低昆虫的适应性.
昆虫肠道微生物群在昆虫生理学和健康方面发挥着至关重要的作用.
益生菌可以恢复肠道微生物群,改善无菌昆虫的健康状况.

研究的目的:

调查益生菌联盟 (乳糖菌,肠杆菌和Klebsiella oxytoca - LEK-PC) 在20代的绝育地中海果 (Ceratitis capitata) 适应性的影响.
分析LEK-PC对肠道微生物群结构和无菌的代谢功能的影响.

主要方法:

建立了地中海果 (V8-GSS) 的四个殖民地:控制 (C),幼虫+益生菌 (L+),成年人+益生菌 (A+),以及幼虫和成年人+益生菌 (AL+).
益生菌联盟 (LEK-PC) 给幼虫和/或成人的饮食.
评估了适应性参数 (出现,飞行,应激生存,交配竞争力) 和肠道微生物群 (16S rRNA测序).

主要成果:

在无菌中中,LEK-PC的使用显著改善了出现,飞行能力,应激生存和交配竞争力,无论施用阶段如何.
16S元基因组学揭示了肠道微生物群结构的重大变化,核心社区不受LEK-PC管理的影响.
代谢功能的预测显示,AL+殖民地碳水化合物,氨基酸,辅因子和维生素代谢的丰富.

结论:

使用LEK-PC进行益生菌治疗有效地恢复和调节无菌地中海果的肠道微生物群.
这种益生菌干预增强了关键的健康参数,提高了无菌昆虫技术对害虫管理的有效性.

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