在液体染色体质谱分析系统中,N-尼托拉胺在列上的微量形成
James R Wickens1, Andrew P Dominey1
1Medicinal Science and Technology, GlaxoSmithKline R&D, Stevenage, UK.
Rapid communications in mass spectrometry : RCM
|January 11, 2024
概括
在di-n-butylamine和酸盐的分析过程中,在LC-MS系统中形成了微量N-nitrosamine. 这种化发生在柱上,影响了灭和未灭样本之间的分析结果.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 有机化学 有机化学
背景情况:
- 尼托胺是一种具有潜在健康风险的化合物.
- 精确量化微量N-尼托拉胺对于环境和食品安全监测至关重要.
- 现有的分析方法可能容易在样本处理过程中形成人工物.
研究的目的:
- 在使用LC-MS分析di-n-butylamine和酸盐时研究N-nitrosamines的形成.
- 在分析工作流程中识别人工N-尼托胺形成的来源和机制.
- 了解柱上化对定量结果的影响.
主要方法:
- 采用了使用di-n-butylamine和酸盐的模型反应系统.
- 液体染色学-质谱学 (LC-MS) 用于分析.
- 进行了涉及灭和未灭样本的动力学研究.
- 进行了控制实验,涉及将样品引入自取样器针中.
主要成果:
- 在灭和未灭的样本之间观察到系统的度偏移,这表明在分析过程中形成了N-尼托拉胺.
- 确切地说,N-尼托拉胺的形成与LC-MS系统有关,特别是发生在柱上.
- 实验设置证实,在自动采样器针内混合氨酸和酸盐导致了酸胺生成.
结论:
- LC-MS系统,特别是柱上环境,可以成为人工N-尼托拉胺形成的来源.
- 分析程序必须考虑列上的潜在化,以确保精确的微量N-胺量化.
- 这一发现凸显了用于涉及反应性物种的微量分析方法验证的重要性.
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