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应对挑战:优化用于初级细胞培养分离的方法.

Oliwia Piwocka1,2,3, Marika Musielak4,5,6, Karolina Ampuła7

  • 1Department of Electroradiology, Poznan University of Medical Sciences, Poznan, 61-701, Poland. oliwia.piwocka@wco.pl.

Cancer cell international
|January 11, 2024
PubMed
概括

这项研究提出了一种有效的方法,用于隔离初级乳腺癌细胞培养物,使用 hialuronidase 和 collagenase. 这种技术有助于克服纤维细胞过度生长等挑战,使得癌症研究更容易获得初级细胞系.

关键词:
乳腺癌 乳腺癌是什么?癌细胞 癌细胞 癌细胞与癌症相关的纤维细胞.酶性消化过程中的酶性消化隔离 隔离 隔离 隔离主细胞系的主要细胞系.初级文化是一种初级文化.

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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