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基于代谢途径,探索骨髓瘤中代谢和免疫微环境之间的关系

Changwu Wu^1,2, Jun Tan^3,4, Hong Shen^5,6,7

¹Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Journal of biomedical science

|January 11, 2024

概括

代谢途径,特别是维生素和辅助因子代谢,显著影响骨髓瘤.

科学领域:

* 瘤学瘤学是一门专业.
* 分子生物学 * 分子生物学
* 免疫学免疫学

背景情况:

*代谢重编程在骨髓瘤中至关重要,影响预后和治疗结果.
* 瘤新陈代谢与瘤免疫微环境 (TIME) 之间的相互作用需要进一步阐明.

研究的目的:

* 为了研究代谢途径与骨髓瘤的TIME之间的关系.
* 识别预测治疗反应和患者预后的代谢特征.
* 探索关键代谢相关基因在骨髓瘤进展中的功能作用.

主要方法:

*分析了来自TARGET和GEO数据库的RNA-Seq和临床数据 (n=84).
*推断代谢超级通路活动和免疫细胞透.
*无监督的聚类,以识别代谢和代谢相关基因 (MRG) 聚类.
*基于MRG的风险模型的构建和验证.
* 分子实验评估ST3GAL4在骨髓瘤细胞中的功能.

主要成果:

* 维生素和辅助因子代谢与骨髓瘤预后和时间有很强的相关性.
*两个不同的代谢 (C1,C2) 显示出差异性的免疫透和化疗反应 (C2优异).
*三个MRG群 (GC1-3) 呈现出不同的预后和免疫透水平;GC3预测了对免疫疗法和化疗更好的反应.
* 一个经过验证的代谢相关风险模型显示出预后预测能力,低风险组显示出更高的免疫透率.
* ST3GAL4敲击抑制了骨髓瘤细胞的增殖,入侵,糖解和M2巨细胞的两极分化.

结论:

* 维生素和辅助因子代谢是骨髓瘤时间和预后的关键调节者.
*MRG集群有效地反映了 TIME 的变化,并预测了对化疗和免疫疗法的反应.
* 开发的新陈代谢相关风险模型显示出对骨髓瘤的预后预测有希望.
*ST3GAL4与骨髓瘤的进展,糖解和TIME的调制有关.

关键词:

代谢过程中的代谢.骨质肉瘤骨质肉瘤是什么预测预后预测预测 ST3GAL4 德国人对治疗的反应反应.瘤的免疫微环境维生素和辅助因子维生素和辅助因子

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