Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proofreading01:43

Proofreading

54.1K
Overview
54.1K
Mismatch Repair01:36

Mismatch Repair

40.1K
Overview
40.1K
Base Excision Repair01:54

Base Excision Repair

22.4K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.4K
Overview of DNA Repair02:25

Overview of DNA Repair

31.0K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
31.0K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.6K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

NanoSimFormer: an end-to-end transformer-based nanopore signal simulator with basecaller guidance.

Bioinformatics (Oxford, England)·2026
Same author

Epstein-Barr virus-associated lymphoepithelioma-like intrahepatic cholangiocarcinoma with concurrent hepatitis B virus infection: case report and literature review.

Annals of medicine and surgery (2012)·2026
Same author

A Case of Nonpedal Subcorneal Haematoma Mimicking Melanoma.

Acta dermato-venereologica·2026
Same author

RAS mutation status and immune microenvironment define distinct prognostic landscapes and predict chemotherapy benefit in pMMR colorectal cancer.

Frontiers in immunology·2026
Same author

Decoding the role of chromatin context in the off-target effects of CRISPR gene editing with EGOLD.

Cell discovery·2026
Same author

PLANeT: Understanding and leveraging the genome of land plants for a sustainable future.

Cell·2026
Same journal

Post-Moore two-dimensional integrated electronics for angstrom-nodes.

National science review·2026
Same journal

A multienzyme-mimicking nanoplatform induces disulfidptosis/cuproptosis/apoptosis for tumor therapy.

National science review·2026
Same journal

Nanogalvanic cell catalysts: bridging electrochemical and thermal catalysis.

National science review·2026
Same journal

Temporal genomics reveal rapid adaptation to pesticide exposure in Eastern honeybees.

National science review·2026
Same journal

Making reservoirs cleaner through a Pattern-Process-Effect-Regulation framework.

National science review·2026
Same journal

Occupancy as a key attribute linking saprotrophic fungi to soil carbon decomposition.

National science review·2026
查看所有相关文章

相关实验视频

Updated: Jul 5, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K

通过软决策解码,提高DNA数字存储中的纠错能力.

Lulu Ding1, Shigang Wu1, Zhihao Hou1,2

  • 1Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen518120, China.

National science review
|January 12, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种用于DNA数字存储 (DDS) 的新型软决策解码方法,增强了错误纠正而不添加冗余. 德里克系统显著提高了各种测序技术的存储容量和可靠性.

关键词:
DNA数字存储 (DDS) 是一种数字存储系统.错误纠正能力的错误纠正能力.错误纠正代码 (ECC) 是一个错误纠正代码.软决策解码的解码方法储存量 储存量 储存量 储存量

更多相关视频

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K

相关实验视频

Last Updated: Jul 5, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K

科学领域:

  • 生物技术是生物技术.
  • 信息科学 信息科学 信息科学
  • 计算机科学 计算机科学

背景情况:

  • 当前的DNA数字存储 (DDS) 系统面临错误纠正代码 (ECC) 的局限性,平衡错误纠正与数据冗余.
  • 在DDS的一个关键挑战是提高错误弹性,而不会增加存储开销.

研究的目的:

  • 为DDS引入软决策解码方法,以克服纠错能力和冗余性之间的权衡.
  • 开发一个DNA特定的错误预测模型和新的策略,以提高DDS性能.

主要方法:

  • 提出了一个软决策解码方法,集成到Reed-Solomon (RS) 编码的DDS系统中,命名为Derrick.
  • 开发了一种特定于DNA的错误预测模型和新的解码策略.
  • 通过在体外和在体内的实验与Illumina,PacBio和牛津纳米孔技术 (ONT) 测序验证了该方法.

主要成果:

  • 德里克系统在没有额外冗余的情况下显著提高了纠错能力.
  • 在ONT测序的体外实验表明,德里克将RS代码的纠错能力翻了一番,并将解码失败减少了229倍.
  • 该系统将潜在的最大存储容量扩大了32,388倍,在信息密度和序列深度效率方面超过了最先进的DDS系统.

结论:

  • 拟议的软决策解码策略为DNA数字存储提供了实质性的进展.
  • 德里克的方法提高了存储容量和可靠性,优于现有的DDS方法.
  • 软决策解码策略和德里克的核心组件可以将其推广到其他ECC解码算法.