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Updated: Jul 5, 2025

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

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从TCGA数据库中探索头部和部状细胞癌中的预后免疫微环境相关基因

Shuangjiang Li¹, Yiyu Zeng², Liming He¹

¹Department of Stomatology, Changsha Stomatological Hospital, Changsha, P. R. China.

Journal of Cancer

|January 12, 2024

概括

这项研究确定了与转移和患者存活相关的头支状细胞癌 (HNSCC) 中的关键瘤微环境 (TME) 基因. 这些与TME相关的基因可以作为HNSCC的预后和转移性标记物.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
生物信息学是一种生物信息学.

背景情况:

头部和部状细胞癌 (HNSCC) 经常转移,影响患者的治疗结果.
瘤微环境 (TME) 在HNSCC的发展和进展中发挥着关键作用.
了解TME基因相互作用对于预测HNSCC转移模式至关重要.

研究的目的:

在HNSCC中选TME相关基因.
识别与免疫和 stromal 细胞透相关的差异表达基因 (DEGs).
评估这些DEG在HNSCC中的预后和转移性意义.

主要方法:

使用表达数据 (ESTIMATE) 算法对恶性瘤中的STromal和免疫细胞进行估计.
分析了TCGA HNSCC的基因表达,纯度和树皮/免疫透的数据.
进行了差异基因表达分析,蛋白质-蛋白质相互作用网络分析和生存分析.

主要成果:

免疫和ESTIMATE得分与HNSCC预后有显著的相关性.
确定了433个上调和394个下调的DEG与ESTIMATE和免疫评分相关.
生存分析强调了CCR4,CXCR3,P2RY14,CCR2,CCR8和CCL19作为与免疫透和转移相关的显著预后标志物.

结论:

使用ESTIMATE选了与TME相关的基因,并在HNSCC中确定了与生存相关的DEG.
这些已识别的基因标记物对于预测HNSCC的预后和转移潜力非常有价值.
这些发现提供了关于TME在HNSCC转移中的作用的见解,并建议潜在的治疗点.

关键词:

估计估计估计免疫评分免疫评分免疫评分部状细胞癌瘤树分数是什么?瘤微环境是一个微环境.

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