相关实验视频

Updated: Jun 28, 2026

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

适应性控制的E. 根据氧气吸收率,养批量种植中的大肠杆菌特异生长率

Renaldas Urniezius¹, Deividas Masaitis¹, Donatas Levisauskas¹

¹Department of Automation, Kaunas University of Technology, Studentu 48, LT-51367 Kaunas, Lithuania.

Computational and structural biotechnology journal

|January 12, 2024

概括

这项研究引入了细胞生物质特异生长率 (SGR) 的自动控制系统,用于食批量培养. 该系统使用氧气吸收率 (OUR) 测量进行自适应控制,在模拟和实验中证明可行性.

科学领域:

生物技术是生物技术.
过程控制过程控制
生物反应器工程生物反应器工程

背景情况:

在生物工艺中,食批量种植对于最大限度地提高产品产量至关重要.
控制细胞生物质特异生长率 (SGR) 对于优化料批处理过程至关重要.
现有的方法可能会与生物过程固有的时间变化的条件作斗争.

研究的目的:

开发一种自动控制系统,用于精确控制SGR的设置点.
使用氧气吸收率 (OUR) 测量进行实时SGR估计和反控制.
调整控制系统以适应动态操作条件,以提高性能.

主要方法:

开发一个具有适应能力的比例整合 (PI) 控制器.
整合基于在线OUR测量的SGR估计器.
通过数值模拟和实验室规模的料批生物反应器实验进行验证.
通过开放循环数字双 SGR 估计器和自适应 SGR 控制进行交叉验证.

主要成果:

开发的控制系统成功地跟踪了所需的生物质生长概况.
在线OUR测量使控制器能够适应不断变化的工艺条件.
与SGR估计器相比,数字双胞胎估计器在统计上显示的偏差较小.
模型预测控制表现出卓越的稳定性,在特定场景中显著优于自适应系统.

结论:

拟议的自动控制系统提供了一种可行的方法,用于SGR设定点控制料批培养.
基于OUR测量的自适应控制提高了过程稳定性和性能.
对先进的控制策略,如模型预测控制等进行进一步的调查是有必要的.

关键词:

适应性控制是适应性的控制.在Fed-batch文化中.设定点控制控制器设置点控制器具体增长率的具体增长率是什么

更多相关视频

Precise, High-throughput Analysis of Bacterial Growth

Precise, High-throughput Analysis of Bacterial Growth

Published on: September 19, 2017

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

相关概念视频

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Methods for Controlling Microbial Growth

Methods for Controlling Microbial Growth

Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...

Bioreactor Controls-I

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Designing Growth Media for Bioreactors

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

Fed-Batch Culture

Fed-Batch Culture

Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...