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上游开放阅读框架介导的ANAC082表达的上调,以应对Arabidopsis中的核子应激
Shun Sasaki1, Toru Murakami2, Miharu Yasumuro2
1Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
Plant biotechnology (Tokyo, Japan)
|January 12, 2024
概括
在阿拉比多的ANAC082上游开放阅读框架 (uORF) 在核应激过程中增强了ANAC082的表达. 这种依赖于uORF的调节是特定于核细胞应激,而不是内质网膜应激,并且在ANAC103进化过程中丢失了.
科学领域:
- 植物分子生物学 植物分子生物学
- 细胞应激反应细胞应激反应
- 基因调节 基因调节
背景情况:
- 核糖体生物发生扰乱引发核应激,激活植物的适应反应.
- 阿拉比多普西斯ANAC082转录因子是植物核细胞应激反应的关键调节者.
- ANAC082mRNA具有一个5'-未翻译区域 (5'-UTR) 与一个上游开放的读取框架 (uORF).
研究的目的:
- 研究ANAC082 uORF在核应激期间调解基因表达变化的作用.
- 为了确定ANAC082 uORF是否对内质网膜 (ER) 应激有反应.
- 为了比较ANAC082 uORF的调节功能与其同类ANAC103.3的调节功能.
主要方法:
- 使用的转基因阿拉比多普西斯表达了在ANAC082促进体和5'-UTR.下露西法酶记者.
- 应用于核应激诱导试剂和ER应激诱导试剂进行记者基因测试.
- 在ANAC082和ANAC103.3中进行过渡表达试验以评估uORF功能.
主要成果:
- 在诱导核应激时,记者基因表达以uORF-依赖的方式被上调.
- ANAC082 uORF没有调解ER应激反应.
- 与ANAC082 uORF不同的是,ANAC103 uORF没有表现出依赖序列的抑制作用.
结论:
- ANAC082 uORF对阿拉比多普西斯菌中核应激反应至关重要.
- ANAC082的uORF-依赖调节是特定于核子应激,而不是在ER应激下观察到的.
- 进化差异导致ANAC103同类物体失去依赖uORF的调节.
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