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Updated: Jul 5, 2025

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兰化物复合增强生物对等分支DNA扩增

Fang Zhao1, Yunpeng Guan2, Fei Su2

  • 1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Analytical chemistry
|January 12, 2024
PubMed
概括

兰化物复合增强生物对等分支DNA放大 (LEBODA) 在单细胞中提供高度敏感的in situ核酸检测. 这种新的方法显著改善了用于检测罕见核酸标的信号放大.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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