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两种不同物种之间的DNA转移是通过Clostridium共同培养中异性细胞融合进行的

Kamil Charubin¹, John D Hill¹, Eleftherios Terry Papoutsakis¹

¹Department of Chemical and Biomolecular Engineering, The Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.

|January 12, 2024

概括

在Clostridium共同培养中,异质细胞融合使得DNA交换成为可能,从而产生具有集成等离子体DNA的混合细胞. 这扩大了对微生物组进化和生存策略的理解.

科学领域:

微生物学微生物学
进化生物学进化生物学
遗传学遗传学是一个

背景情况:

Prokaryotic 进化依赖于突变和水平基因转移 (HGT).
之前的研究表明,Clostridium acetobutylicum和Clostridium ljungdahlii共同培养中的异质细胞融合促进了蛋白质和RNA的交换.
HGT机制包括转换,转导和结合.

研究的目的:

调查C. acetobutylicum和C. ljungdahlii之间的异质细胞融合是否促进DNA交换.
确定混合微生物群落中遗传物质获取和整合的机制.
探索产生具有新型遗传性质的混合微生物细胞的潜力.

主要方法:

合成培养C. acetobutylicum和携带等离子体的C. ljungdahlii菌株.
选择性亚培养,以分离获得等离子体DNA的细胞.
用于DNA甲基化分析和基因组整合评估的PacBio单分子实时 (SMRT) 测序.
限制稀释涂层以确认混合细胞的存在.

主要成果:

隔离了C. acetobutylicum细胞,这些细胞从C. ljungdahlii.获得并集成了来自C. ljungdahlii.的等离体DNA.
在C. acetobutylicum基因组中发现了红红素抗性 (erm) 基因的马赛克式集成的证据.
根据PacBio SMRT测序数据,可以证明C. acetobutylicum/C. acetobutylicum混合物的存在. 具有混合DNA甲基化模式的ljungdahlii细胞.

结论:

异质细胞融合是C. acetobutylicum和C. ljungdahlii之间DNA交换的机制.
这一过程可以导致混合细胞与集成的外来DNA形成,扩展微生物基因组.
这些发现提高了对微生物组动态,适应和潜在的生物技术应用的理解.

关键词:

克洛斯特里迪亚姆乙甲基 (Clostridium acetobutylicum) 是一种制剂.克洛斯特里 (Clostridium ljungdahlii) 是一种有毒的植物.交换 DNA 交换 DNA 交换 DNA 交换 DNA整合 DNA整合 DNA整合不同类细胞的融合.塑体 DNA DNA 的 DNA .综合性缩是什么意思

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