相关实验视频

Updated: Jul 5, 2025

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

编码假定抗CRISPR类基因的Shewanella菌体代表了一个新的潜在病毒家族

Hongmin Wang¹, Kaiyang Zheng¹, Min Wang^1,2,3,4

¹College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, MOE Key Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, China.

Microbiology spectrum

|January 12, 2024

概括

一种新的Shewanella菌体,vB_SbaS_Y11,被分离和鉴定. 这种菌体含有未表征的抗CRISPR基因,可能代表一种新的病毒家族,Ranviridae.

科学领域:

微生物学微生物学
病毒学病毒学
基因组学就是基因组学.

背景情况:

谢瓦内拉菌对海洋生物地化学循环至关重要.
很少有Shewanella菌被隔离和表征.
舍瓦内拉菌体在全球分布在各种水生环境中.

研究的目的:

为了隔离和表征一种新的Shewanella菌体.
研究新菌体的基因组特征和分类学分类.
为了解Shewanella菌体多样性和分布做出贡献.

主要方法:

从污水中分离了谢瓦尼拉菌体vB_SbaS_Y11.
传输电子显微镜用于形态学.
基因组测序和分析.
遗传学和比较基因组分析.

主要成果:

vB_SbaS_Y11 拥有双面角头部和长尾部.
它的线性,双链DNA基因组为62,799 bp,其中有71个ORF.
发现了一种非特征化的抗CRISPR AcrVA2基因.
遗传学分析表明vB_SbaS_Y11代表了一个潜在的新病毒家族,Ranviridae.

结论:

vB_SbaS_Y11是一种具有独特基因组特征的新型Shewanella菌体.
对抗CRISPR AcrVA2基因的发现扩大了对菌体与宿主相互作用的知识.
vB_SbaS_Y11和相关菌体可能形成一个新的病毒家族,Ranviridae,增强对菌体多样性的理解.

关键词:

牧羊人群中的一个.谢瓦内拉菌体菌体这是一种抗CRISPR的AcrVA2抗体.基因组和比较基因组分析.遗传学分析遗传学分析

更多相关视频

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Genome Size and the Evolution of New Genes

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.