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在莱姆病病原体中,BadR直接抑制了糖醇利用操作子的表达

Jun-Jie Zhang¹, Sajith Raghunandanan¹, Qian Wang²

¹Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.

Journal of bacteriology

|January 12, 2024

概括

莱姆病细菌Borrelia burgdorferi使用糖醇作为能量. 研究人员发现了一种特定的DNA区域和一种称为BadR的蛋白质,它们共同控制和抑制细菌使用甘油的能力,这对于不同宿主的生存至关重要.

科学领域:

微生物学和分子生物学
病原体的适应和调控

背景情况:

Borrelia burgdorferi的糖利用对于莱姆病的发病和载体内的生存至关重要.
负责甘油代谢的glpFKD操作子的表达受到包括循环二-GMP (阳性) 和RpoS (阴性) 在内的因素的严格调节.

研究的目的:

确定控制B. burgdorferi. glpFKD操作子表达的新型调控元素.
阐明Borrelia宿主适应调节器 (BadR) 在glpFKD调节中的作用.

主要方法:

基因查以确定影响glp表达的DNA结合蛋白.
对BADR调节器的过度表达和突变分析.
电泳运动转移试验 (EMSAs) 证实直接BADR与glp cis元素的结合.

主要成果:

在glp的5'未翻译区域中发现了一种新的cis调节元件,它调节负调节.
过度表达BadR显著抑制了glp表达,而badR突变显示了增加的glp表达.
BadR直接与已识别的cis元素结合,抑制了独立于RpoS的glp表达.

结论:

BadR通过与特定的cis元素结合,作为glpFKD操作子的直接抑制剂.
B. burgdorferi使用涉及BadR和RpoS的多层调节机制来控制糖的利用.
了解这些调节通路是解读病原体适应和宿主特异性基因表达的关键.

关键词:

波雷莉亚的形虫 (Borrelia burgdorferi) 是一种形虫.莱姆病是莱姆病的一种疾病.糖醇代谢的代谢过程一个小小的.

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