孕期训练的状NK细胞可以保护孕妇免受有害的Fusobacterium nucleatum感染
Rebecca Kotzur1, Shira Kahlon1, Batya Isaacson1
1The Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada, The Hebrew University Hadassah Medical School, Jerusalem, Israel.
PLoS pathogens
|January 12, 2024
概括
孕期训练的叶NK细胞 (PTdNKs) 通过分泌更多的花粉素,为胎儿提供了针对Fusobacterium nucleatum感染的增强的胎儿保护. 这一发现突显了PTdNKs在怀孕期间对抗细菌威胁方面的新作用.
科学领域:
- 免疫学 免疫学 免疫学
- 生殖生物学 生殖生物学
- 微生物学 微生物学
背景情况:
- 在母胎界面的自然杀手 (NK) 细胞 (dNK) 对于怀孕成功至关重要.
- 在第一次怀孕后,dNKs经历"教育",成为经过怀孕训练的dNKs (PTdNKs),功能得到改善.
- PTdNK与更好的胎盘和胎儿生长有关.
研究的目的:
- 为了研究PTdNKs对Fusobacterium nucleatum (FN) 感染的保护作用.
- 阐明 PTdNK 赋予保护的机制.
- 在体内模型中验证保护作用.
主要方法:
- 将PTdNKs中的FN诱导的粒素素 (GNLY) 分泌量与第一次怀孕中的dNKs进行比较.
- 研究FN的Fap2蛋白和PTdNKs的Gal-GalNAc之间的分子相互作用.
- 使用花素转基因 (GNLY-tg) 的小鼠在体内评估胎儿对FN的保护.
主要成果:
- 与第一次怀孕的dNK相比,PTdNK在FN挑战时分泌的花素 (GNLY) 含量显著更高.
- 增加PTdNKs的GNLY分泌会导致FN的杀死.
- FN的Fap2蛋白与PTdNK表达的Gal-GalNAc之间的相互作用调解了GNLY的分泌.
- 与依赖NK细胞的野生类型小鼠相比,GNLY-tg小鼠表现出更好的胎儿防范FN感染.
结论:
- 孕期训练的状NK细胞 (PTdNKs) 具有增强的保护胎儿免受细菌感染的能力.
- PTdNKs通过增加的花素分泌,通过细菌Fap2与Gal-GalNAc的相互作用触发,对Fusobacterium nucleatum进行保护.
- 这项研究揭示了PTdNK在母胎界面的新型免疫保护功能.
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