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分析调节ZmEREB24如何改善玉米 (Zea mays) 苗木干旱耐受性的分子机制

Zhenzhen Ren¹, Jiaxu Fu², Salah Fatouh Abou-Elwafa³

¹College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.

Plant physiology and biochemistry : PPB

|January 12, 2024

概括

一个关键的基因,ZmEREB24,增强了玉米幼苗的干旱耐受性. 过度表达提高了弹性,而绝杀增加了灵敏度,突出了其在作物育种干旱适应中的作用.

科学领域:

植物科学植物科学
遗传学遗传学是一个
农业科学农业科学

背景情况:

干旱压力严重限制了玉米的生产力,特别是在苗阶段.
提高苗木对干旱的耐受性对于玉米繁殖至关重要.
AP2/ERF转录因子家族对于植物非生物应激反应至关重要.

研究的目的:

鉴定和描述玉米中对干旱压力反应的AP2基因.
研究ZmEREB24在灌木阶段赋予干旱耐受性的作用.
阐明ZmEREB24介导干旱适应的基础分子机制.

主要方法:

转录组分析以确定ZmEREB24.24的身份.
产生ZmEREB24过度表达和淘汰 (CRISPR/Cas9) 的玉米系.
在干旱压力条件下的表型评估.
通过RNA-seq,电泳运动转移试验 (EMSA) 和DNA亲和力净化测序 (DAP-seq) 来识别目标基因和结合部位.

主要成果:

过度表达ZmEREB24显著提高了转基因玉米苗的干旱耐受性.
ZmEREB24淘汰突变体表现出增加的干旱敏感性.
欧洲环境安全局和RNA-seq确定了AATGG.CT和GTG.T.GCC动机作为ZmEREB24的结合部位.
DAP-seq揭示了四种新的基因,这些基因参与了proline/sugar代谢和激素信号传递.

结论:

在播种阶段,ZmEREB24在调节玉米干旱耐受性方面发挥着至关重要的作用.
ZmEREB24通过与向基因促进体结合并调节它们的表达而起作用.
ZmEREB24是一种有希望的候选基因,用于高产,耐旱的玉米品种的分子育种.

关键词:

在AP2基因中,AP2基因被发现.干旱造成的压力是干旱.玉米玉米玉米是一种这就是ZmEREB24的意思.

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