将触觉传感器与爆炸性质标记信号放大策略集成在一起,用于使用微型质谱仪进行超敏感和多重分析
Ying Zhang1, Linsen Li2, Jingjing Li3
1Key Laboratory of Consumer Product Quality Safety Inspection and Risk Assessment for State Market Regulation, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Biosensors & bioelectronics
|January 12, 2024
概括
这项研究引入了用于超敏感雌激素检测的新型聚合诱导排放光原体 (AIEgen) 标记质量探测器. 吸收传感器平台提供了显著的信号放大,并有可能在现场对痕迹分析物的多重分析.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 带有aptamers和质量标签的质量探测器可以进行敏感的,特定的多重检测.
- 高效的质量标签释放对于在食感器开发中的信号放大至关重要.
研究的目的:
- 开发聚合诱导排放光原体 (AIEgen) 标记质量探针 (AIEMPs) 用于雌激素分析.
- 使用纳米脂质体将一个食感器与爆炸性质量标签信号放大策略集成在一起.
- 为了实现对雌激素的超敏感和多重检测.
主要方法:
- 通过混合化aptamer修饰的Fe3O4纳米粒子和AIEgen负载的纳米脂质体组装AIEMP.
- 超声波辅助乳化用于纳米脂质体制备.
- 电子喷雾沉积和纳米电子喷雾电离质谱仪 (nanoESI-MS) 用于信号检测和放大.
主要成果:
- 基于AIEMP的食欲传感器实现了超敏感的雌激素检测,检测极限为0.168-0.543 pg/mL.
- 与直接的纳米ESI-MS相比,证明了四个数量级的信号放大.
- 通过使用不同的AIEMP和微型质谱仪实现了三种雌激素的多重检测.
结论:
- 开发的AIEMP平台为例行MS检测微量分析剂提供了一种多功能方法.
- 这项工作扩展了大规模标记策略,并显示了现场检测应用的潜力.
- 吸食传感器对雌激素分析具有高灵敏度,特异性和复杂化能力.
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