转录因子的共同表达调解了胚胎和胚胎外分化的血统原始化
Alba Redó-Riveiro1, Jasmina Al-Mousawi1, Madeleine Linneberg-Agerholm1
1reNEW UCPH - The Novo Nordisk Foundation Center for Stem Cell Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
Stem cell reports
|January 12, 2024
概括
研究人员确定了一个独特的胚胎干细胞 (ESC) 群体,共同表达SOX2和GATA6. 这一发现揭示了早期哺乳动物发育和细胞命运的决定.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 早期哺乳动物发育涉及胚胎和胚胎外转录因子在芽细胞和内部细胞质量 (ICM) 细胞中的共同表达.
- 胚胎干细胞 (ESC) 通常表达胚胎因子,但观察到一个独特的群体共同表达非胚胎因子.
研究的目的:
- 确定和描述ESCs共同表达胚胎因子SOX2和胚胎外因子GATA6.
- 调查这一独特的ESC亚群的差异化潜力和转录格局.
- 分析SOX2和GATA6在增强剂元素的结合模式,了解它们的合作作用.
主要方法:
- 利用基于蛋白质的双报告器系统来识别共同表达SOX2和GATA6.6的ESC.
- 执行单细胞转录组学来分析已识别的ESC群体的特征.
- 在ESC和外胚胎内皮干细胞中映射增强元件.
- 在ICM类ESC亚群中评估了SOX2和GATA6对这些增强元素的结合.
主要成果:
- 确定了一种新的ESC群体,共同表达SOX2和GATA6,类似于未分离的ICM.
- 这种类似于ICM的ESC群体显示出增强的内皮分化潜力,同时保持了表皮质能力.
- SOX2和GATA6在增强剂中表现出合作结合;GATA6与SOX2结合多能增强剂,而SOX2与GATA6.6广泛结合内皮增强剂.
结论:
- 在ESC中SOX2和GATA6的共同表达反映了具有双重差异化潜力的非分离ICM状态.
- SOX2和GATA6在增强剂的合作结合对于ESC自我更新和为内皮分化做准备至关重要.
- 这一发现为管理哺乳动物发育早期细胞命运决定的调节机制提供了洞察力.
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