含有黄素的单氧化酶 (FMO1-6) 的分子和功能表征在树中
Yasuhiro Uno1, Miaki Makiguchi2, Genki Ushirozako1
1Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima-city, Kagoshima 890-0065, Japan.
概括
确定和描述了含有树黄素的单氧化酶 (FMO). 树FMO3表现出与人类FMO3相似的功能,表明其在药物代谢研究中的实用性.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 基因组学就是基因组学.
背景情况:
- 含有黄素的单氧化酶 (FMO) 是人类中重要的药物代谢酶.
- 树,一个非动物灵长类的物种,被用于生物医学研究,但其FMO仍然没有特征.
- 研究树FMO可以揭示它们在药物代谢研究中的潜力.
研究的目的:
- 隔离和描述树含有黄素的单氧化酶 (FMO1-6).
- 与人类FMO相比,分析树FMO的序列身份,遗传关系和组织表达.
- 用特定的基质评估树FMO的代谢功能.
主要方法:
- 树FMO1-6cDNAs的分离和测序. 树FMO1-6cDNAs的分离和测序. 树FMO1-6cDNAs的分离和测序. 树FMO1-6cDNA的分离和测序.
- 序列分析,包括与人类FMO进行身份比较.
- 遗传学分析以确定进化关系.
- 在各种树组织中对FMO mRNA表达的定量分析.
- 用N-氧化和S-氧化试验对复合树FMO蛋白的功能性表征.
主要成果:
- 树FMOcDNA和氨基酸序列与人类FMO具有很高的相同性 (85-90%和81-89%).
- 遗传学分析显示,树和人类FMO之间存在密切的集群,这表明保存的基因结构.
- FMO3和FMO1mRNA分别在肝脏和脏中最为丰富.
- 所有重组树FMO1-6蛋白都表现出胺N-氧化和甲基p-托利硫化物S-氧化活性.
- 树 FMO3 主要代谢的三甲基胺,一个选择性的人类FMO3基质.
- 树FMO6显示了三甲基胺活性,与人类FMO6P伪基因不同.
结论:
- 树FMO1-6基因与人类的FMO1-5和FMO6P是正义的.
- 树FMO3具有与人类FMO3相比的功能和分子特征,是肝脏的主要FMO.
- 已识别的树FMO,特别是FMO3,适合进一步的药物代谢研究.
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