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来自巨型病毒的异醇酸激酶的生物化学和结构特征

Guangning Zong¹, Yann Desfougères², Paloma Portela-Torres²

¹Inositol Signaling Group, Signal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, 27709, USA.

The EMBO journal

|January 12, 2024

概括

巨型病毒编码了新型的内醇酸盐激酶 (IPK),这些激酶可以合成内醇酸盐 (PP-IP). 结构和生物化学研究揭示了TvIPKK.

科学领域:

病毒学病毒学
生物化学生化学
结构生物学结构生物学
细胞信号传输细胞信号传输

背景情况:

内醇酸盐 (IPs) 和铁酸盐 (PP-IPs) 是真核细胞中至关重要的信号分子,调节各种生物过程.
细胞信号分子的剧目正在扩大,包括病毒成分.
核细胞质大DNA病毒 (NCLDVs) 具有复杂的基因组,并与宿主细胞机械广泛相互作用.

研究的目的:

从生物化学和结构上表征Terrestrivirus,一种NCLDV的类型的内醇酸盐激酶 (TvIPK).
阐明TvIPK功能的酶活性,基质特异性和结构基础.
探索病毒IPKs在伊诺西酸铁酸盐信号传递的背景下对其进化的影响.

主要方法:

生物化学试验以确定TvIPK的酶活性和基质范围.
采用X射线晶体学,以获得TvIPK与基质和核酸复合的高分辨率结构.
现场定向突变发生,以调查保存残留物在催化和结构中的作用.
在酵母细胞中表达TvIPK以研究其在真核细胞系统中的功能.

主要成果:

在体外和在酵母中,TvIPK从各种螺旋和肌酸酸盐中合成异醇铁酸盐.
晶体结构揭示了一个心形的连接体结合口袋,灵活的,充电的残留物容纳了各种基质.
在G循环中保存的阿基尼因对于引导ATP的马酸盐用于酸化至关重要.
突变性研究证实了保存的催化和建筑特征的重要性.

结论:

TvIPK代表了一个极简的内醇酸盐激酶,具有广泛的基质特异性,其独特的活性站点架构使其成为可能.
编码为NCLDV的IPK可能在伊诺西铁酸盐信号通路的演变中发挥了作用.
在土壤环境中,TvIPK可能会对土壤病毒存在的土壤环境产生重大生物地质化学影响.

关键词:

细胞信号传递细胞信号传递巨大的病毒病毒.伊诺西酸伊诺西酸基因酶激酶的使用方法酸盐地质化学酸盐地化学

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