呼吸道疾病降低了上皮干细胞的治疗潜力
Lisa Zhang1,2, Natalie Kelly3, Kimberly M Shontz4
1Department of Otolaryngology-Head and Neck Surgery, The Ohio State Wexner Medical Center, Columbus, OH, USA.
Respiratory research
|January 12, 2024
概括
气道疾病可能会影响用于组织工程气道移植 (TETG) 的人类气管支架上皮质 (hTBE) 细胞的再生潜力. 儿科患者的某些临床因素与状基底细胞表型有关,影响表皮再生.
科学领域:
- 再生医学是一种再生医学.
- 组织工程是组织工程.
- 肺部医学 肺部医学
背景情况:
- 组织工程气管移植 (TETG) 为呼吸道重建提供了一个潜在的解决方案.
- 通过宿主集成或与自身细胞进行预播种,可以实现TETG的再细胞化.
- 预先存在的呼吸道疾病对TETG上皮质细胞再生能力的影响尚不清楚.
研究的目的:
- 调查呼吸道疾病是否会改变人类气管支气管上皮质 (hTBE) 细胞的再生潜力.
- 评估来自患者的hTBE细胞的能力,以支持TETG再细胞化.
主要方法:
- 一项队列研究涉及48名儿科和6名接受临床指示支气管镜检查的成年患者.
- 人类气管支气管上皮质 (hTBE) 细胞是通过粘膜刷毛获得的.
- 细胞增殖和分化潜力在第1和第2段被评估为重新细胞化能力.
主要成果:
- 基底细胞恢复和频率在不同年龄和不同解剖区域都是一致的.
- 在通道2的所有样本都产生了足够的细胞来进行3.5x0.5cm2的脚手架的高密度重细胞化.
- 儿科和成人样本的一个子集表现出状基底细胞表型,该表皮极化和分化受损,并且与早产,显著的肺病史 (包括支气管肺功能失调) 和儿科患者的先前气管切除术有关.
结论:
- 对于TETG的hTBE细胞的再生潜力受到呼吸道疾病的影响,特别是在高危儿科人群中.
- 鉴定临床风险因素和特定的细胞表型可以帮助选择使用气管支架进行呼吸道重建的合适候选者.
- 基于临床病史和实验室发现的患者查对于优化气管组织工程的结果至关重要.
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