通过包装在外膜囊泡中的细菌小RNA抑制宿主植物防御
Yan Wu1, Sai Wang1, Peihong Wang1
1Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Shanghai Cooperative Innovation Center for Modern Seed Industry, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Plant communications
|January 13, 2024
概括
植物病原性细菌使用外膜囊泡 (OMV) 将小RNA (sRNA) 输送到米植物中,如Xosr001. 这种细菌sRNA抑制宿主免疫力,增强疾病.
科学领域:
- 植物与微生物的相互作用
- 细菌外膜囊泡 (OMVs) 是一种细菌外膜囊泡.
- 小RNA (sRNA) 的生物学
背景情况:
- 在OMV中的细菌sRNA调解物种间的通信,但它们在植物病原体相互作用中的作用基本上是未知的.
- 植物病原性细菌利用OMV将分子送入宿主植物,可能影响疾病的发展.
- 了解OMV介导的植物病原体的分子机制对于开发疾病控制策略至关重要.
研究的目的:
- 来自植物病原体Xanthomonas oryzae pv. 的OMV中的差异包装sRNA的特征. 奥里兹科拉 (Xoc).
- 为了研究一个特定的sRNA (Xosr001) 在调节宿主植物免疫力中的功能.
- 阐明Xoc OMV将Xosr001输送到大米中的机制及其对疾病的影响.
主要方法:
- 用RNA测序来识别包装在Xoc OMV中的sRNA.
- 使用定量分析和体内测试来确定Xosr001在调节OsJMT1表达和MeJA积累中的作用.
- 使用转基因大米线和细菌感染试验的功能研究评估了Xosr001对口腔免疫和疾病易感性的影响.
主要成果:
- 一个候选sRNA,Xosr001,被发现在Xoc OMVs中很丰富,并且特别针对大米叶组织.
- Xosr001有效地抑制了米中的OsJMT1转录积累,导致MeJA水平降低.
- 过度表达OsJMT1的转基因大米系表现出减少的口腔免疫力和增加对缺乏Xosr001的Xoc的敏感性,证实了Xosr001在免疫抑制中的作用.
结论:
- 通过Xoc OMVs传递的Xosr001,通过抑制大米口腔免疫功能作为一种毒性因子.
- 这项研究揭示了一种新的王国间通信机制,其中细菌sRNA操纵宿主防御途径.
- 针对OMV介导的sRNA传递可能为控制大米中的细菌病提供新的策略.
更多相关视频
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
21.8K
08:25Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
Published on: March 20, 2016
12.7K
相关概念视频
02:26Defenses Against Pathogens and Herbivores
23.7K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
23.7K
01:23Types of RNA
63.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.7K
01:31Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:30siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K