Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Modular <i>in vitro</i> evaluation of buparlisib-polymeric nanomedicines in 2D and 3D models of glioblastoma.

Frontiers in toxicology·2026

Same author

Recent advances in fluorescent probes for guided surgery of head and neck carcinomas.

RSC advances·2026

Same author

Functional divergence and structural changes of Class IV histone deacetylases (HDACs) across the tree of life.

Molecular biology and evolution·2026

Same author

The influence of structural diversity in polymer-based nanomedicines on anti-cancer efficacy.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

Polymeric Theranostics with Tetraphenylporphyrin for Effective Low-Dose Photodynamic Cancer Therapy.

Pharmaceutics·2026

Same author

Current state of knowledge on the epidemiology, aetiology, diagnosis, and management of heart involvement in eosinophilia. A Clinical Consensus Statement of the ESC Working Group on Myocardial & Pericardial Diseases.

European journal of heart failure·2026

Search research articles

相关实验视频

Updated: Jul 5, 2025

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

基于的聚合物多功能成像工具用于生物化学.

Anuson Sansee¹, Libor Kostka², Adéla Marcalíková³

¹Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.

|January 13, 2024

概括

研究人员使用探针开发了一种新的宏分子成像工具,用于生物研究. 这种工具可以选择性地标记表达谷氨酸碳酸酶II (GCPII) 的细胞,并为增强成像提供长时间的光寿命.

关键词:

这就是HPMA的HPMA.这就是iBody.影像成像技术影像成像技术复合物复合物复合物

更多相关视频

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

相关实验视频

Last Updated: Jul 5, 2025

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

科学领域:

生物结合化学生物结合化学
宏分子科学宏分子科学
生物成像是一种生物成像.

背景情况:

开发先进的分子探针对于精确的生物研究至关重要.
现有的成像工具往往缺乏特异性或最佳的光物理性质.
宏分子平台提供多功能支架,用于集成多种功能.

研究的目的:

为生物应用创建一个宏分子多功能成像工具.
为了整合一个以为基础的发光探针,一个GCPII准配体,和一个生物素标签.
评估开发的成像工具在各种生物测试中的性能.

主要方法:

合成基于N-(2-基) 甲基胺的宏分子探针.
一个三环金属复合物的功能化用于结合.
探针在流动细胞计 (FACS),激光共聚焦显微镜和FLIM中的应用.
选择性向谷氨酸碳酸酶II (GCPII) 表达细胞.

主要成果:

开发了具有不同聚合物结构和复合物含量的大分子探针.
使用FACS和共聚焦显微镜对表达GCPII的细胞进行选择性标记.
通过FLIM观察到标记探针的光寿命超过100 ns,将其与有机光体区分开来.
证实了iBody概念在目标细胞成像中的实用性.

结论:

开发的宏分子成像工具表现出选择性的细胞向和独特的长光寿命特性.
基于的探头在特定的生物成像应用中比传统的有机光体具有优势.
该研究强调了iBody概念和发射器对未来生物工具开发的潜力.