相关实验视频

Updated: Jul 5, 2025

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

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适应研究RNA依赖性转化调节在黄金葡萄球菌的研究的核糖体分析方法

Maximilian P Kohl1, Béatrice Chane-Woon-Ming1, Roberto Bahena-Ceron1

  • 1Architecture et Réactivité de l'ARN, CNRS 9002, Université de Strasbourg, Strasbourg, France.

Methods in molecular biology (Clifton, N.J.)
|January 13, 2024
PubMed
概括

这项研究适应了核糖体概况,以了解小调节性RNA (sRNA) 如何控制金黄色葡萄球菌中的基因表达. 这种方法提供了对转化调节的全基因组视图,这对于细菌适应和致病性至关重要.

关键词:
里博-雷特 (Ribo-RET) 是一个城市.里博-seqq 的意思核糖体造型分析 核糖体造型分析黄金葡萄球菌黄金葡萄球菌开始编码子.开始翻译开始翻译翻译条例 翻译条例

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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Regulation of Expression Occurs at Multiple Steps

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Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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