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Updated: Jul 5, 2025

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
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对小RNA稳定性和目标调节的5'-末端酸化在体外的评估

Alexandra Schilder1,2, Yvonne Göpel1,3, Muna Ayesha Khan1,3

  • 1Department of Microbiology, Immunobiology and Genetics, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC), Vienna, Austria.

Methods in molecular biology (Clifton, N.J.)
|January 13, 2024
PubMed
概括

研究人员开发了一种新的工具来研究具有不同5'端的细菌小RNA (sRNA). 该系统允许按需生成5'单酸化sRNA (5'P),用于体内研究.

关键词:
5′末端酸化的方法5′单酸盐的使用方法5′三酸盐的使用方法体核糖核酶RNaseE的使用这就是GLMZ的意思.在RNA衰变过程中,RNA衰变.处理RNA处理RNA的过程拉普Z拉普Z拉普Z小RNA (sRNA) 是一种小RNA.

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