Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

Search research articles

相关实验视频

Updated: Jul 5, 2025

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

使用等离子体图书馆对大肠杆菌中sRNA标的定向查.

Xing Luo¹, Nadim Majdalani²

¹Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD, USA. xing.luo@nih.gov.

Methods in molecular biology (Clifton, N.J.)

|January 13, 2024

概括

研究人员开发了一种新的方法来创建和选细菌小调节性RNA (sRNA) 表达库. 这项技术有助于识别控制大肠杆菌中特定基因的sRNA调节器,从而提高我们对基因调节的理解.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Precision medicine decision-making under pharmacogenomics in nursing: realizing value from limited evidence to scaled clinical pathways.

Frontiers in neurology·2026

Same author

Integrative and interpretable machine learning framework for early non-invasive detection of clinically significant liver fibrosis.

Frontiers in medicine·2026

Same author

PDIA2 promotes progression of lung cancer by regulating JNK and caspase-3 signaling pathway.

Gene·2026

Same author

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same author

Case Report: pharmaceutical care in a case of complicated urinary tract infection combined with disseminated <i>Nocardia brasiliensis</i> infection.

Frontiers in medicine·2026

Same author

Cucurbitacin B induces immunogenic cell death and activates adaptive immunity in cutaneous melanoma via the ROS-STAT3-eIF2α/IRE1 pathway.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

科学领域:

细菌学细菌学是一门学科.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

细菌的小调节RNA (sRNA) 对于基因表达控制至关重要.
sRNAs通过基配对来调节翻译和mRNA稳定性.
确定特定的sRNA-基因相互作用是细菌研究中的一个重大挑战.

研究的目的:

介绍一个构建sRNA表达式库的协议.
为了展示这个库用于选sRNA调节者的使用.
为了促进识别控制大肠杆菌中特定基因的sRNAs.

主要方法:

开发一个创建多样化的sRNA表达库的协议.
使用sRNA库实施查策略.
该方法的应用用于在大肠杆菌中识别sRNA调节剂.

主要成果:

成功构建了一个功能性的sRNA表达库.
展示图书馆在对基因调节sRNA的查中的实用性.
对特定感兴趣的基因进行潜在的sRNA调节者的鉴定.

结论:

描述的协议提供了一种有效的方法来识别细菌sRNA调节器.
sRNA表达库是研究转录后基因调节的宝贵工具.
该协议可适应用于其他细菌物种.

关键词:

湖Z融合湖Z融合他们是sRNARNA.在sRNA图书馆的屏幕上

更多相关视频

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

相关实验视频

Last Updated: Jul 5, 2025

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012