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相关概念视频

Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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相关实验视频

Updated: Jul 5, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

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使用CLASH定义细菌RNA-RNA相互作用体

Sofia Esteban-Serna1, Liang-Cui Chu1, Mehak Chauhan1

  • 1Centre for Engineering Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.

Methods in molecular biology (Clifton, N.J.)
|January 13, 2024
PubMed
概括

这项研究详细介绍了一种优化的CLASH协议,用于分析宿主感染期间甲素耐药黄金葡萄球菌 (MRSA) 中的RNA-RNA相互作用. 这些发现揭示了小RNAs (sRNAs) 在MRSA中的关键调节作用.

关键词:
质量控制分析质量控制分析在RNA-RNA相互作用中.紫外线交叉连接的交叉连接

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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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相关实验视频

Last Updated: Jul 5, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 甲素耐药黄金葡萄球菌 (MRSA) 导致严重的感染,死亡率高.
  • 小RNAs (sRNAs) 是已知的S. aureus中抗生素耐药性和毒性的调节者.
  • 大多数sRNA在MRSA宿主感染中的特定作用在很大程度上没有被描述.

研究的目的:

  • 在模仿宿主条件下研究MRSA中的RNA-RNA互动组.
  • 提出一个优化的CLASH协议,用于研究GRAM阳性细菌中的sRNA与宿主相互作用.
  • 开发用于分析RNA-RNA相互作用和识别sRNA-mRNA调节关系的计算工具.

主要方法:

  • 混合体的交叉链接,结合和序列化 (CLASH) 适应了黄金色.
  • 在模拟宿主环境的条件下进行MRSA体外培养.
  • 在CLASH数据中开发和应用Python函数来分析RNA折叠能量和种子区域.

主要成果:

  • 在宿主感染期间在MRSA中生成了RNA-RNA相互作用的全面地图.
  • 建立了一个优化的CLASH协议,适用于S. aureus和其他グラム阳性细菌.
  • 提供了计算工具,以方便分析sRNA介导的基因调节.

结论:

  • 这项研究提供了一种强大的方法来剖析MRSA中的sRNA调节网络.
  • 了解这些相互作用对于开发针对MRSA感染的新疗法至关重要.
  • 开发的计算工具可以从CLASH数据中标准化评估RNA-RNA相互作用.