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转录因子LBD16针对桃子侧根形成中的细胞壁修饰/离子运输基因

Xuelian Wu¹, Zhe Wang¹, Anqi Du¹

¹College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, China.

Plant physiology

|January 13, 2024

概括

侧面器官边界域/非对称叶子2-Like (LBDs/ASLs) 是关键的植物转录因子. 这项研究揭示了PpLBD16的存在.

科学领域:

植物分子生物学植物分子生物学
植物开发植物开发
遗传学是一个遗传学.

背景情况:

侧面器官边界域/非对称叶子2-Like (LBDs/ASLs) 是植物特有的转录因子,调节侧面根 (LR) 的形成.
之前的研究发现PpLBD16是桃子 (Prunus persica) LR形成的积极调节者.
下游调节网络和PpLBD16的基因在很大程度上仍然没有被描述.

研究的目的:

为了阐明PpLBD16的下游监管网络.
在根部发育过程中识别由PpLBD16调节的直接点基因.
了解PpLBD16在控制桃子侧根器官生成中的作用.

主要方法:

在桃色的PpLBD16同类过度表达和沉默线的构建.
基因组基因组测序 (RNA-seq) 分析来自修改系的根组织.
用DNA亲和性净化测序 (DAP-seq) 来识别PpLBD16结合因子和部位.
综合分析RNA-seq和DAP-seq数据以确定候选目标基因.

主要成果:

过度表达PpLBD16促进了桃子根的启动,而沉默则抑制了它.
通过RNA-seq识别了参与细胞壁修饰,离子运输和平衡的PpLBD16调节基因.
DAP-seq显示PpLBD16与特定的DNA基因 (MEME-1,MEME-2,ABR1) 结合在一起.
已经证明PpLBD16可以结合和激活关键基因:PpEXPB2,PpSBT1.7,PpCNGC1和PpPPO,控制根器官生成.

结论:

PpLBD16直接调节参与细胞壁修饰,离子运输和平衡的基因.
PpLBD16在桃子侧根原生菌的发育和形成中起着至关重要的作用.
这项研究揭示了PpLBD16的下游分子网络,进步了我们对LBD16介导的横向根发育的理解.

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