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在Prdm9的指编码异构体中的自然变异会影响小鼠中的混合不育现象型
Khawla F N AbuAlia1, Elena Damm1, Kristian K Ullrich1
1Research Group Meiotic Recombination and Genome Instability, Max Planck Institute for Evolutionary Biology, Plön D-24306, Germany.
Genetics
|January 13, 2024
概括
在小鼠中,生殖隔离与PRDM9基因有关. 特定的等位基因,dom2和msc1,导致杂交不育,这表明野生小鼠群体中罕见的不育等位基因.
科学领域:
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
- 生殖生物学 生殖生物学
背景情况:
- 通过PRDM9介导的生殖隔离最初在Mus musculus杂交品种中观察到.
- 混合男性不孕症是由Prdm9基因和Hstx2基因之间不兼容引起的,破坏了半变异.
研究的目的:
- 调查野生小鼠群体中新型PRDM9等位基因在生殖隔离中的作用.
- 为了确定特定的PRDM9等位基因是否诱导跨亚种类型的老鼠杂交中的不育性.
主要方法:
- 在欧洲,亚洲和中东的野生老鼠种群中测序Prdm9外因子12.
- 在小鼠中分析Prdm9等位基因,这些小鼠具有进发的t-哈普类型.
- 在外交野生种群和具有特定Prdm9等位基因组合的同系菌株中评估杂交不孕.
主要成果:
- 在野生小鼠群体中确定了14个Prdm9等位基因,其中包括8个新基因.
- DOM起源的dom2等位基因和MUS msc1等位基因的组合导致杂交品种完全不孕.
- 在Mus musculus种群中观察到PRDM9结合基因的删除,可能与Prdm9等位基因不结合.
结论:
- 这项研究证实,PRDM9介导的杂交不育性超出了同系老鼠菌株的范围.
- 特定的PRDM9等位基因组合 (dom2和msc1) 对于跨子基因的混合不孕症至关重要.
- 在野生老鼠群体中,PRDM9的无菌等位基因似乎很罕见.
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