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在精液样本中使用DNA甲基化Y染色体CpG进行年龄预测

Ji Eun Lee¹, Sang Un Park¹, Moon Hyun So¹

¹Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, the Republic of Korea.

Forensic science international. Genetics

|January 13, 2024

概括

对Y染色体标记物的DNA甲基化分析可以预测精液样本中的年龄. 这种方法有助于在涉及混合体液的性侵犯案件中缩小嫌疑人池.

科学领域:

法医科学法医科学法医科学
遗传学遗传学是一个
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

性侵犯案件通常涉及混合生物样本 (男性和女性DNA).
Y染色体短并列重复 (Y-STR) 在识别来自同一父系的嫌疑人时有用但有限.
年龄估计可以在法医调查中显著缩小嫌疑人的识别范围.

研究的目的:

开发和验证基于DNA甲基化的年龄预测模型,使用Y染色体标记.
评估这些模型在法医案例工作中的实用性,特别是混合DNA样本.

主要方法:

通过Illumina的MethylationEPIC BeadChip Array对56名韩国男性的精液样本进行DNA甲基化分析.
在Y染色体上识别与年龄相关的CpG (cytosine-phosphateg-guanine) 位点.
在147个精液样本的多重检测数据上开发使用各种回归分析和支持矢量机的年龄预测模型.

主要成果:

在Y染色体上确定了10个与年龄相关的CpG标记物.
用九个标记器构建年龄预测模型,在测试样本上达到57年的平均绝对误差 (MAE).
多重检测证明了可靠性,可重复性和灵敏性;女性样本显示微不足道的干扰.

结论:

Y染色体DNA甲基化分析为男性的年龄估计提供了可靠的方法.
开发的年龄预测模型对于法医应用是有效的,特别是在性侵犯案件中混合性别样本中.
这种技术提供了一种有价值的工具,可以通过根据年龄对嫌疑人进行歧视来帮助调查.

关键词:

年龄年龄年龄年龄年龄年龄大规模并行测序.甲基化甲基化甲基化精子精子精子精子这就是Y染色体.

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