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通过基于纳米孔的测序来确定布拉格废水中SARS-CoV-2变种的流行率

Alžběta Dostálková¹, Kamila Zdeňková², Jana Bartáčková³

¹Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic; National Institute of Virology and Bacteriology, University of Chemistry and Technology Prague, Czech Republic.

|January 13, 2024

概括

废水监测有效地比临床数据更早地检测到SARS-CoV-2变种,为公共卫生提供了关键的工具. 这种早期预警系统有助于预测即将到来的COVID-19浪潮和城市人口中的变种流行率.

科学领域:

环境微生物学环境微生物学
病毒学病毒学
公共卫生监督是对公共卫生的监督.

背景情况:

基于废水的流行病学 (WBE) 是一种经过验证的方法来监测像COVID-19这样的传染病.
在废水中早期检测SARS-CoV-2变种可以预测疾病爆发,并为公共卫生响应提供信息.
了解城市人口的变异动态对于管理COVID-19大流行至关重要.

研究的目的:

评估布拉格废水中SARS-CoV-2变体和亚变体的存在和进展.
为了比较废水和临床样本之间的变异检测时间.
分析不同城市地区的SARS-CoV-2血统动态的空间变化.

主要方法:

在废水样本中基于纳米孔的SARS-CoV-2RNA测序.
来自布拉格各个下水道区的废水样本的分析.
将WBE数据与临床监测数据进行比较.

主要成果:

废水监测检测到SARS-CoV-2RNA拷贝增加,此前临床检测了三角洲变种浪潮.
在临床样本中占主导地位大约一个月后,新的SARS-CoV-2变种出现在废水中.
在布拉格的下下流域中观察到变种流行动态的显著差异,与人口密度和流动性相关.

结论:

废水监测为SARS-CoV-2变种的出现和传播提供了早期预警系统.
WBE可以识别变体进展的局部差异,帮助针对性干预.
这种方法提高了针对病毒性疾病的积极公共卫生战略的能力.

关键词:

流行病学流行病学基于纳米孔的测序.布拉格布拉格布拉格布拉格布拉格布拉格布拉格这就是SARS-CoV-2病毒.变种变种变种变种废水是废水,废水就是废水.

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