由各种氨源抑制无氧消化,导致代谢物动态的微妙差异
Xiaoqing Wang1, Stephany Campuzano1, Angéline Guenne1
1Université Paris-Saclay, INRAE, PRocédés biOtechnologiques au Service de l'Environnement, 92761, Antony, France.
Chemosphere
|January 13, 2024
概括
伴随氨的阳离子通过影响微生物代谢,显著改变无氧消化. 代谢学和高级统计学揭示了不同的离子,如酸盐,如何影响代谢途径和短链脂肪酸降解.
科学领域:
- 环境微生物学环境微生物学
- 生物化学 生物化学
- 分析化学是一种分析化学.
背景情况:
- 氨对无氧消化的影响已经得到了充分的研究,但伴随性离子的作用往往被忽视.
- 了解这些影响对于优化无氧消化过程和生物气生产至关重要.
研究的目的:
- 研究氨盐中存在的不同离子对无氧消化性能和微生物代谢动态的影响.
- 为了证明代谢学与时间流程统计框架相结合的有用性,用于分析复杂的生物数据.
主要方法:
- 利用液态染色体质谱法 (LC-MS) 和双重质谱法对9个时间点的实验室规模批量反应堆样本进行代谢分析.
- 应用过程序来选择重要的代谢特征,然后进行时间过程建模,聚类和差异分析.
- 采用代谢物注释来识别分子结构和阐明不同阴离子所影响的代谢途径.
主要成果:
- 确定了与不同离子处理相关的不同代谢物动态.
- 证明,离子,如酸盐,可以减缓特定代谢物的消耗,如5-氨基瓦勒酸,一种l-lysine降解的产物.
- 代谢峰值的聚类揭示了不同受实验条件影响的生物过程之间的相关性.
结论:
- 代谢学与统计框架相结合,有效地捕捉了不同条件下的代谢物动态的微妙差异,为微生物过程提供了深入的见解.
- 这项研究强调了阴离子对无氧消化过程的显著,但经常被忽视的影响,提供了超出氨直接影响的更全面的理解.
相关概念视频
01:20Overview of Nitrogen Metabolism
7.9K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.9K
00:46Feedback Inhibition
53.9K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
53.9K
01:30The Electron Transport Chain
16.8K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.8K
01:18Indirect-Acting Cholinergic Agonists: Mechanism of Action
1.8K
Indirect-acting cholinergic agonists work by interacting with an enzyme called acetylcholinesterase (AChE) in the synaptic cleft. They can be reversible or irreversible inhibitors and have different effects on the enzyme.
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex,...
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex,...
1.8K
01:29Drugs Affecting Neurotransmitter Synthesis
1.4K
Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase,...
1.4K