来自棕色海藻Tubinaria decurrens的fucoidan:结构和结构 - 抗癌活性关系
Anh Ngoc Nguyen1, Quang Van Ngo2, Thu Thi Minh Quach2
1Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Viet Nam.
International journal of biological macromolecules
|January 13, 2024
概括
这项研究揭示了来自Turbinaria decurrens的富科伊丹的复杂结构,证明了它对结肠癌的强大抗癌活性. 降低分子量和优化结构可以增强富可伊丹.
科学领域:
- 海洋天然产品 化学 化学
- 碳水化合物化学 碳水化合物化学
- 癌症生物学 癌症生物学
背景情况:
- 富科伊丹是棕色海藻中发现的硫酸多糖,具有多种生物活动.
- 轮 (Turbinaria decurrens) 是一种棕色海藻物种,具有潜在的制药应用.
- 了解富可伊丹的结构对于优化其治疗疗效至关重要.
研究的目的:
- 为了阐明来自Turbinaria decurrens的fucoidan的详细结构.
- 为了评估这种富可伊丹对HT29人类结肠癌细胞的抗癌活性.
- 为了确定福科丹抗癌作用的结构-活性关系.
主要方法:
- 使用多角度激光光散射 (SEC-MALLS) 进行尺寸排除色谱.
- 红外光谱学 (IR) 红外光谱学
- 电子喷雾电离质谱仪 (ESI-MS) 是一种电子喷雾电离质谱仪.
- 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学
- 小角度X射线散射 (SAXS) 是一种
- 用HCl进行水解.
主要成果:
- 富科伊丹 (Mw 122.6 KDa) 具有 (1→3) -和 (1→4) -α-L-Fucp残留的骨干,具有特定的硫化模式和β-D-Galp分支.
- 原生和水解的福科丹在溶液中表现出类似杆状的形状 (Rg c:0.531.52 nm).
- 富可伊丹对HT29细胞具有显著的抗癌活性 (IC50:5.41±0.36至73.52±2.54μg/mL).
结论:
- 来自T. decurrens的Fucoidan具有与抗癌性质相关的定义复杂结构.
- 通过降低分子量和实现特定的棒状结构,抗癌活性显著增强.
- 优化福科伊丹结构,以小分子量和短枝为特征,是有效结肠癌治疗的关键.
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