甲基化程度:对于将icaritin与pectin封装成一个关键因素
Yipeng Chen1, Tiantian Zhao2, Lina Cheng2
1Key State Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
pectin中甲基化 (DM) 的程度显著影响其封装药物的能力. 乳纳米载体中较高的DM增强了药物载荷能力,并改善了小细胞形成,以改善药物输送.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 纳米技术 纳米技术
背景情况:
- pectin是一种天然的多糖,具有作为药物输送的纳米载体的潜力.
- 甲基化 (DM) 的程度是影响pectin物理化学特性的关键性质.
- 素的DM对其对治疗剂的封装能力的特定影响尚未完全理解.
研究的目的:
- 为了研究点的甲基化程度 (DM) 对icaritin的封装效率和负载能力的影响.
- 用不同的DM水平制备的点纳米载体 (菌体) 的物理化学特性.
- 阐明点丁DM与伊卡里/点丁小粒 (IPM) 的形成和稳定性之间的关系.
主要方法:
- 制备低甲基化pectin (LMP) 和高甲基化pectin (HMP) 与受控的DM水平.
- 素分子量和质性质的表征.
- 使用LMP和HMP.两种方法制备的伊卡里/pectin micelles (IPMs) 的配方.
- 对负载能力,粒子大小,Zeta潜力和多分散率指数的IPM分析.
主要成果:
- 与LMP-IPM (15.72-16.64%) 相比,在HMP-IPM (18.75-20.12%) 中观察到更高的负载能力.
- 随着DM的增加,LMP-IPM的颗粒大小下降 (449-527nm),而HMP-IPM则在DM水平更高 (342-566nm) 时显示出更小的颗粒大小.
- 在DM和IPM形成参数之间发现了正相关性,包括封装效率,负载能力,Zeta潜力和多分散度指数.
结论:
- pectin的甲基化 (DM) 程度是影响其作为icaritin纳米载体的性能的一个关键因素.
- 乳中的更高的DM提高了IPM的封装效率和负载能力.
- 这些发现表明,可以优化点的DM,以控制基于点的药物递送系统的形成和特性.
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