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Updated: Jul 5, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

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在光相关谱学中对自相关函数进行统计分析

John Kohler1, Kwang-Ho Hur1, Joachim Dieter Mueller2

  • 1School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota.

Biophysical journal
|January 14, 2024
PubMed
概括

光相关谱 (FCS) 的新装配方法通过准确估计不确定性和解决复杂的生物样本来改善数据分析,克服传统技术的局限性.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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