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基于线性溶解能量关系模型的芳香静止相的合成和评估,用于超临界流体色谱的扩展应用

Dandan Ge¹, Jie Yang¹, Zimo Yu¹

¹Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.

Journal of chromatography. A

|January 14, 2024

概括

为超临界流体染色学 (SFC) 合成了四种新型芳香静止相. 这些相提供可调的π-π,静电和键相互作用,增强复杂分子的分离,如黄类和类.

科学领域:

分析化学分析化学
染色体学染色体学是一种染色学.

背景情况:

超临界流体色谱 (SFC) 使用芳香静止相进行分离.
开发新的静止相对于推进色谱技术至关重要.

研究的目的:

为了合成和描述四种基于SFC的完全多孔粒子 (FPP) 的新型芳香静止相.
用线性溶解能量关系 (LSER) 模型研究这些阶段的保留机制.
评估静态相特性和移动相添加剂对分离性能的影响.

主要方法:

在FPP上合成四种芳香静止相 (S-aniline,S-1-ami-naph,S-1-ami-anth,S-1-ami-py).
应用线性溶解能量关系 (LSER) 模型来分析保留机制.
研究相互作用,包括键,π-π,双极-双极和阴离子交换.
定量分析了离子交换能力 (d+值) 和SFC添加剂 (酸,三酸,酸,氨基酸,甲基胺) 之间的关系.
通过优化移动相组合,证明了对黄类药物的改善峰形状和对异类类药物的成功化.

主要成果:

合成的芳香静止相呈现中等极性,并提供 π-π,键,二极管二极管和离子交换相互作用的组合.
LSER分析显示,连接体类型和结合密度在一定程度上影响了这些相互作用.
与商业化列相比,S-1-ami-anth列显示出更高的阴离子交换能力.
移动相添加剂显著调节了阴离子交换相互作用,允许控制静电相互作用.
优化的添加剂度改善了黄类药物的峰值形状,并促进了异类类素的化.

结论:

开发的芳香静止相为SFC提供了独特的π-π和可控制的静电相互作用.
这些阶段显示出在SFC中扩大应用的巨大潜力,特别是用于分离复杂混合物.
该研究强调了通过固定相设计和移动相优化来理解和控制保留机制的重要性.

关键词:

芳香的静止阶段芳香的静止阶段线性溶解能量的关系.保留机制的保留机制是什么超临界流体色谱学超临界流体色谱学

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