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Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

664
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
407
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

485
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
485
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

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This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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相关实验视频

Updated: Jul 5, 2025

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
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吸附异温的参数逐参数估计方法在疏水性相互作用色谱中.

Yu-Xiang Yang1, Yu-Cheng Chen1, Shan-Jing Yao1

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, China.

Journal of chromatography. A
|January 14, 2024
PubMed
概括

一种新的参数对参数 (PbP) 方法简化了疏水性相互作用色谱 (HIC) 模型的参数估计. 这种方法通过系统地确定HIC参数,减少复杂性来加速生物制药工艺开发.

关键词:
疏水性相互作用色谱学 疏水性相互作用色谱学机械模型是机械模型.莫勒鲁普是一个热热体.参数估计的参数估计.

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科学领域:

  • 生物技术是生物技术.
  • 化学工程是化学工程的重要组成部分.
  • 染色体学 染色体学是什么

背景情况:

  • 疏水性相互作用色谱 (HIC) 对于生物制药净化至关重要.
  • 目前的HIC过程开发是耗时的,模型参数估计效率低下.
  • 机械模型为工艺表征和设计提供了潜力.

研究的目的:

  • 为HIC机械模型开发一个高效和逻辑的参数估计策略.
  • 提出一个参数对参数 (PbP) 方法来估计Mollerup异热参数.
  • 为了减少HIC过程开发所需的复杂性和时间.

主要方法:

  • 一种新的参数对参数 (PbP) 方法是基于理论推导和简化假设而开发的.
  • PbP方法涉及三个步骤:线性回归 (LR),线性近似 (LA) 和反向方法.
  • LR估计盐-蛋白相互作用和平衡常数;LA估计石基度参数和最大结合能力;反向方法估计蛋白-蛋白相互作用和动力系数.

主要成果:

  • PbP方法系统地单独估计HIC参数,将逆估计参数数量从6个减少到2个.
  • 该研究确定了LR (稀释) 和LA (过渡/非线性区域) 的最佳条件.
  • 通过数值实验和现实世界的应用,验证了PbP-HIC方法,增强了机械学的理解.

结论:

  • PbP方法为估计HIC模型参数提供了一个系统和有效的策略.
  • 这种方法加速了HIC过程的开发,并改善了机械学的理解.
  • PbP方法显示了对其他模型的应用潜力,这些模型是从静态位移原理衍生的.