强烈的N2固定加速了农田土壤中的微生物周转
Wei Gao1, Jun Zhao2, Xiaobin Guo3
1Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, PR China; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China.
The Science of the total environment
|January 14, 2024
概括
添加有机碳 (葡萄糖) 刺激了土壤中的生物固化 (BNF),主要是由自由生活的亚杆菌 (如Azotobacter) 驱动的. 这个过程支持微生物生长和碳循环,特别是在碳比较高的土壤中.
科学领域:
- 土壤微生物学 土壤微生物学
- 生物地质化学生物地质化学
- 气循环是指气循环的过程.
背景情况:
- 生物固定 (BNF) 对于土壤肥力至关重要,但对碳 (C) 和 (N) 的可用性敏感.
- 酶活性的代谢成本使得BNF对有机C输入的反应及其对微生物周转的影响不清楚.
研究的目的:
- 调查有机碳添加对BNF和农田土壤微生物周转的影响.
- 确定影响葡萄糖诱导BNF的关键微生物参与者和土壤因素.
主要方法:
- 结合15N2跟踪与高通量测序.
- 12天内用葡萄糖或葡萄糖加矿物质N肥料化了三个农田土壤.
- 使用结构方程模型 (SEM) 和随机森林 (RF) 分析.
主要成果:
- 添加葡萄糖显著增加了BNF活性 (0.76-2.51毫克N kg-1 d-1),而矿物N肥完全抑制了它.
- 葡萄糖诱导的BNF支持微生物生长和有机C矿化,与直接N同化相当.
- 与阿佐托巴克特相关的双菌是葡萄糖诱导的BNF的主要驱动因素 (>50%的nifH基因).
- 土壤的C:N比率和阿佐托细菌的丰度是影响BNF的关键因素.
结论:
- 自由生活的亚热菌在土壤微生物的有机碳循环中发挥着至关重要的作用.
- 有机碳输入,特别是在高C:N比率的土壤中,可以刺激BNF并支持微生物活动.
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