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相关概念视频

Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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用于细胞分离的被动微流体装置.

Tianlong Zhang1, Dino Di Carlo2, Chwee Teck Lim3

  • 1College of Mechanical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212100, China.

Biotechnology advances
|January 14, 2024
PubMed
概括
此摘要是机器生成的。

被动微流体细胞分离为隔离特定细胞类型提供了具有成本效益和可扩展的方法. 本综述探讨了无标签细胞分离技术的各种技术,挑战和未来方向.

关键词:
细胞分离 细胞分离混合动力是一个混合动力.聪明的 聪明的 聪明的没有标签的无标签.消极的微流体学 消极的微流体学

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科学领域:

  • 生物技术是生物技术.
  • 微流体学 微流体学
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞分离对于理解细胞过程,疾病机制以及再生医学和诊断中的应用至关重要.
  • 微流体技术已经通过小型化和理解流体动力学来实现细胞分离的先进.
  • 消极的微流体系统,利用通道结构和流体流体学,提供无标签的细胞分离,没有外部力场.

研究的目的:

  • 审查大量利用被动微流体技术进行细胞分离.
  • 讨论各种被动分离策略,包括过,PFF,DLD和惯性微流体.
  • 概述被动微流体细胞分离当前的挑战和未来的前景.

主要方法:

  • 对被动微流体细胞分离策略的审查.
  • 基于分离机制的分类 (过,沉积,粘附,PFF,DLD,惯性,化,粘弹性,混合).
  • 讨论细胞类型,分离标记和商业化.

主要成果:

  • 被动微流体方法为活跃细胞分离提供了具有成本效益和可扩展性的替代方案.
  • 广泛的被动技术 (例如,PFF,DLD,惯性微流体) 用于各种细胞分离应用.
  • 该审查涵盖了特定的细胞类型,标记物和这些技术的商业格局.

结论:

  • 被动微流体细胞分离是一个快速发展的领域,具有显著的潜力.
  • 需要进一步的研究来应对当前的挑战,并释放未来的发展.
  • 本综述旨在传播知识,指导细胞分离的未来研究和应用.