基于蛋白质多糖的双网微珠改善了胃肠道模型 (TIM-1) 中Bifidobacterium infantis ATCC 15697的稳定性
Wahab Ali Khan1, Masood Sadiq Butt2, Iqra Yasmin3
1Department of Food Science and Technology, University of Home Economics Lahore, 54660 Pakistan.
International journal of pharmaceutics
|January 14, 2024
概括
乳清蛋白分离剂和豆蛋白分离剂的组合可以提高益生菌封装效率和胃肠道中的存活率. 这种基于蛋白质的微封装保护Bifidobacterium infantis,提高存储和运输期间的生存能力.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物材料工程 生物材料工程
背景情况:
- 益生菌的活力对疗效至关重要,常常受到严重的胃肠道疾病的限制.
- 微封装是通过胃肠道 (GIT) 过境期间保护益生菌的关键策略.
- 基于蛋白质的材料具有作为益生菌有效封装剂的潜力.
研究的目的:
- 评估乳清蛋白分离物 (WPI),豆蛋白分离物 (PPI) 和它们的复合物作为Bifidobacterium infantis的微封装剂.
- 确定这些蛋白质矩阵对封装效率 (EE),珠子形态和GIT中的益生菌生存的影响.
- 为了比较不同基于蛋白质的微粒在储存和模拟胃肠道过境期间对益生菌活力的保护作用.
主要方法:
- 使用WPI,PPI和WPI+PPI复合体对Bifidobacterium infantis进行微封装.
- 微珠属性的表征:封装效率,直径和表面形态 (SEM).
- 使用TNO体内胃肠道模型 (TIM-1) 评估益生菌生存率,并在储存28天后评估生命力.
主要成果:
- WPI+PPI复合体产生了最高的封装效率 (94.09%) 并生产了光滑紧的微珠.
- 基于WPI的珠子显示出较低的EE和粗的表面,而PPI珠子则具有带有孔隙的密集表面.
- 封装的益生菌在模拟GIT运输和储存后,与自由细胞 (18.2%的胃,27.5%的肠) 相比,其存活率显著更高 (>10^6-7日志CFU/g).
结论:
- 基于蛋白质的微封装显著提高了GIT中的益生菌生存能力.
- 与单个蛋白质相比,WPI+PPI复合体为Bifidobacterium infantis提供了更好的保护.
- 使用蛋白质复合体的优化微封装策略对于提供功能性益生菌至关重要.
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