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暂时的突触增强是由Drosophila Motoneuron突触突触中的外源供应的单碳酸盐触发的

María-Graciela Delgado¹, Ricardo Delgado¹

¹Department of Biology, Faculty of Sciences, University of Chile, Las Palmeras 3425, 7800001 Santiago, Chile.

|January 14, 2024

概括

酸盐和L-乳酸盐等单碳酸盐暂时增强Drosophila motoneuron神经递质的释放,但无法维持它. 糖糖的添加恢复了释放,突出了葡萄糖分解.

科学领域:

神经科学是一个神经科学.
细胞的新陈代谢
突触生理学突触生理学

背景情况:

质细胞在神经元能量供应中的作用受到争议,最近的研究挑战了天体细胞到神经元的乳酸穿假说.
果神经元输出乳酸,而质细胞可能以脂质滴的形式储存,质疑神经元直接依赖天体细胞产生的乳酸.
外源单碳酸盐对神经元功能,特别是神经递质释放的能量贡献仍然不完全理解.

研究的目的:

调查外部供应的单碳酸盐 (pyruvate和L-乳酸盐) 是否可以支持Drosophila运动神经元中神经递质释放 (NTR).
为了确定在单碳酸供应期间观察到的突触抑郁背后的机制.
探索糖解和六合体在在代谢压力下恢复突触功能的作用.

主要方法:

电生理学记录了电压紧的多索菲拉运动神经元中的刺激后突触电流 (EPSC) 幅度,以评估NTR.
使用FM1-43染料来评估前突触囊泡循环能力.
操纵的突触浴溶液含有不同度的单碳酸盐 (pyruvate,L-乳酸盐) 和碳水化合物 (砂糖).

主要成果:

外源性酸盐和L-乳酸盐最初增强了NTR,但导致了不可恢复的突触抑郁状态.
通过FM1-43负载评估的膀循环在单碳酸盐暴露期间保持完整,将其与突触抑郁脱.
糖糖补充恢复了NTR,表明糖解的关键作用,而单碳酸盐只在糖解严重受限时有所帮助.

结论:

外源单碳酸盐只能为Drosophila motoneuron NTR提供暂时的代谢支持,在持续需求下失败.
由单碳酸盐诱导的突触抑郁并不是由于囊泡循环受损,而是可能与代谢途径限制有关.
糖溶解由糖等六酶提供燃料,对于持续的神经递质释放和突触囊泡招募至关重要,这表明C3碳来源在直接能源供应之外发挥了更广泛的作用.

关键词:

德洛索菲拉运动神经元突触的突触.单碳氧酸单碳氧酸是什么神经递质释放的神经递质释放突触囊泡中的突触囊泡.

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