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相关概念视频

Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...

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相关实验视频

Updated: Jun 13, 2026

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets
13:42

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Published on: November 2, 2011

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在384个井板中进行固相合成.

Mischa Schüttel1, Edward Will1, Gontran Sangouard1

  • 1Institute of Chemical Sciences and Engineering, School of Basic Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Journal of peptide science : an official publication of the European Peptide Society
|January 14, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种高通量合成方法,每次运行可产生1536个短. 这一进步有助于快速选用于类药物发现和其他无需净化应用.

关键词:
有384个井的板块.这就是为什么SPPS是SPPS.组合化学是一种组合化学.类图书馆是一种类图书馆.酸合成的合成

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科学领域:

  • 生物化学 生物化学
  • 化学生物学 化学生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 固相合成 (SPPS) 的进步允许生产高纯度,用于直接的生物活性选.
  • 目前的SPPS仅限于每个微板96个,同时使用多反应器设备并行合成384个.
  • 扩大合成对于药物开发中的全面查至关重要.

研究的目的:

  • 为了修改一个商业合成器384井板兼容性,使更高的吞吐量合成.
  • 为了在一次运行中实现1536个的合成,显著增加了生产能力.
  • 在数量,度和纯度方面验证合成的质量.

主要方法:

  • 用新的硬件组件修改商用合成器.
  • 开发定制软件,以控制384个井板中的合成过程.
  • 1536个短的固体相合成.

主要成果:

  • 一次运行成功合成了1536个短,使用修改合成器和384个井板.
  • 合成的平均产量 (> 0.5 μmol) 和度 (> 10 mM) 高.
  • 获得了不错的平均纯度 (>80%),无需进行染色学净化.

结论:

  • 开发的高通量合成平台使得生产大型库成为可能.
  • 这种方法适用于诸如类药物开发,抗体表位扫描和基质查等应用.
  • 该系统提供了一种具有成本效益和高效的解决方案,用于生成用于生物查的多种类型的组.