高通量N-糖蛋白组学与快速的液体色谱分离
1School of Chemical Science & Engineering, Tongji University, Shanghai, 200092, China.
Analytica chimica acta
|January 14, 2024
概括
一种新的1小时液态色谱法增强了N-糖蛋白组学分析. 这种高通量技术能够在大型样本队列中快速表征N-糖化,加速生物标志物发现.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
- 分析化学 分析化学
背景情况:
- N-糖化是一种关键的翻译后修改,影响蛋白质结构和功能.
- 基于质谱的N-glycoproteomics提供了在完整的N-glycopeptide水平上N-glycosylation的详细表征.
- 临床样本的高通量分析需要更快,更有效的分离技术.
研究的目的:
- 为N-glycoproteomics开发一种快速和高性能的液体色谱梯度方法.
- 为了优化质谱参数以提高吞吐量.
- 应用开发的方法进行大规模的N-糖蛋白质组分析.
主要方法:
- 开发一种为期1小时的液体色谱梯度方法,用于N-糖分离.
- 为快速获取数据,优化质谱参数.
- 该方法用于分析肝细胞癌组织的应用.
主要成果:
- 5,218个完整的N-糖的鉴定.
- 在145种N-糖蛋白上发现了422种特定于位点和结构的差异性N-糖化.
- 证明分析吞吐量大幅增加.
结论:
- 开发的1小时LC-N-glycoproteomics方法显著增加了大规模分析的吞吐量.
- 这种方法适用于高效和快速的N-葡萄糖蛋白质组的特征.
- 这种方法有助于在大型临床队伍中发现生物标志物.
更多相关视频
10:59Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
1.0K
10:37Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
12.0K
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
2.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.0K
01:21Mass Spectrometry: Complex Analysis
785
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
785