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尽量减少CTC识别的错误阳性

Adriana Carneiro¹, Paulina Piairo², Beatriz Matos³

¹International Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga s/n, 4715-330, Braga, Portugal; Experimental Pathology and Therapeutics Group, Research Center of IPO Porto (CI IPOP) / RISE @ CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto), Porto Comprehensive Cancer Center (Porto.CCC), 4200-072, Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS) da Universidade do Porto, Porto, Portugal.

Analytica chimica acta

|January 14, 2024

概括

改善循环瘤细胞 (CTC) 的检测对于癌症监测至关重要. 这项研究引入了一种使用CD15和CD45抗体的双排除方法,通过排除白细胞来准确识别CTC.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

癌症转移是全球死亡的主要原因.
循环瘤细胞 (CTC) 提供实时洞察瘤异质性和进化.
目前的CTC隔离依赖于细胞激素 (阳性) 和CD45 (排除) 生物标志物,但一些白细胞 (WBC) 可能被错误分类.

研究的目的:

为了提高循环瘤细胞 (CTC) 检测和计数的准确性.
制定强有力的排除标准,明确消除干扰性白细胞 (WBC) 种群.
提高CTC在癌症诊断和监测中的临床实用性.

主要方法:

利用免疫细胞化学用于CTC识别.
采用流细胞测量来确认抗体的特异性.
开发了一种结合CD15和CD45抗体的双排斥策略,以区分WBC和CTC.
优化细胞克拉抗体选择,以提高特异性.

主要成果:

确定了表达低CD45和非特异性细胞克拉的粒细胞亚群,导致错误的阳性CTC分类.
证明CD15表达特异地将这些粒细胞识别为白细胞.
通过CD15和CD45双重排除,虚假阳性率从25%降至0.2%.
通过双排斥实现了虚假阳性的完全消除,并改善了细胞克拉抗体的选择.

结论:

颗粒细胞的错误分类对CTC评估准确性产生重大影响.
高性能抗体和双重生物标志物排除提高了CTC检测灵敏度和特异性.
改进的CTC识别有助于更全面的临床应用.

关键词:

循环中的瘤细胞.颗粒细胞颗粒细胞液体活检液体活检微流体学微流体学

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